Comparative Analysis of Virulence and Toxin Expression of Global Community-Associated Methicillin-Resistant Staphylococcus aureus Strains

被引：138
作者：
Li, Min [2 ]
Cheung, Gordon Y. C.
Hu, Jinhui [2 ]
Wang, Decheng [3 ,4 ]
Joo, Hwang-Soo [3 ,4 ]
DeLeo, Frank R. [5 ]
Otto, Michael [1 ]
机构：
[1] NIAID, Lab Human Bacterial Pathogenesis, NIH, Bethesda, MD 20892 USA
[2] Huashan Hosp, Dept Lab Med, Shanghai 200040, Peoples R China
[3] Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Inst Med Microbiol, Shanghai 200433, Peoples R China
[4] Fudan Univ, Shanghai Med Coll, Inst Biomed Sci, Shanghai 200433, Peoples R China
[5] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA
来源：
JOURNAL OF INFECTIOUS DISEASES | 2010年 / 202卷 / 12期
基金：
美国国家卫生研究院; 中国国家自然科学基金;
关键词：
PANTON-VALENTINE LEUKOCIDIN; GENE-EXPRESSION; POLYMORPHONUCLEAR LEUKOCYTES; SKIN INFECTIONS; PNEUMONIA; EVOLUTION; DISEASE; USA300; MRSA; AGR;
D O I：
10.1086/657419
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The current pandemic of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) skin infections is caused by several genetically unrelated clones. Here, we analyzed virulence of globally occurring CA-MRSA strains in a rabbit skin infection model. We used rabbits because neutrophils from this animal species have relatively high sensitivity to Panton-Valentine leukocidin (PVL), a toxin epidemiologically correlated with many CA-MRSA infections. Virulence in the rabbit model correlated with in vitro neutrophil lysis and transcript levels of phenol-soluble modulin a and a-toxin, but not PVL genes. Furthermore, abscesses caused by USA300 and its PVL-negative progenitor USA500 were comparatively large and similar in size, suggesting that PVL has played a limited role in the evolution of USA300 virulence in the context of skin infections. Our study indicates a major but not exclusive impact of virulence on the epidemiological success of USA300 and other CA-MRSA strains and emphasizes the importance of core genome-encoded toxins in CA-MRSA skin infections.
引用
收藏
页码：1866 / 1876
页数：11
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