Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay

被引：19
作者：
Bahlmann, Arnold [1 ]
Falkenhagen, Jana [1 ]
Weller, Michael G. [1 ]
Panne, Ulrich [1 ,2 ]
Schneider, Rudolf J. [1 ]
机构：
[1] BAM Fed Inst Mat Res & Testing, D-12489 Berlin, Germany
[2] Humboldt Univ, Dept Chem, D-12489 Berlin, Germany
来源：
ANALYST | 2011年 / 136卷 / 07期
关键词：
LINKED-IMMUNOSORBENT-ASSAY; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; METABOLITES; IDENTIFICATION; OXCARBAZEPINE; RESIDUE; ELISA; WATER;
D O I：
10.1039/c0an00928h
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples.
引用
收藏
页码：1357 / 1364
页数：8
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