TGF-β/Smad2/3 signal pathway involves in U251 cell proliferation and apoptosis

被引：39
作者：
Zhao, Hong-wei [1 ]
Li, Yan-Wei [2 ]
Feng, Ren [3 ]
Yu, Jian-Bo [4 ]
Li, Jing [5 ]
Zhang, Yan [6 ]
Li, Jin-Cheng [1 ]
Wang, Ya-Xin [6 ]
机构：
[1] Tianjin Med Univ, Dept Anesthesiol, Canc Inst & Hosp, Key Lab Canc Prevent & Treatment Tianjin City, Tianjin 300060, Peoples R China
[2] Tianjin Med Univ, Hosp 2, Dept Nephrol, Tianjin 300211, Peoples R China
[3] Tianjin Huanhu Hosp, Tianjin 300060, Peoples R China
[4] Tianjin Nan Kai Hosp, Dept Anesthesiol, Tianjin 300100, Peoples R China
[5] Tianjin Med Univ Gen Hosp, Dept Anesthesiol, Tianjin 300052, Peoples R China
[6] Tianjin Huanhu Hosp, Dept Anesthesiol, Tianjin 300060, Peoples R China
来源：
GENE | 2015年 / 562卷 / 01期
关键词：
TGF-beta/Smad2/3; Caspase-3; Bcl-2; Apoptosis; RNA INTERFERENCE; CEREBRAL-ISCHEMIA; EXPRESSION; INVASION; BCL-2; TRANSCRIPTION; MECHANISMS; CASPASE-3; PROTEINS; PENUMBRA;
D O I：
10.1016/j.gene.2015.02.049
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
TGF-beta/Smad2/3 signal pathway is regarded as a central regulator in various tumors, but its roles in brain cancer therapy remain unknown. In this study, we identify that the TGF-beta/Smad2/3 signal pathway is activated in human brain glioma cells; inhibitor (SB203580) and siRNA against Smad2/3 quickly inhibited the phosphowlation of Smad2 and 3, expression of its major downstream gene, Ki-67, arrested cells in the G2/M phase and induced apoptosis of cells. The findings suggest that TGF-beta/Smad2/3 pathway plays a key role in U251 cell growth and metastasis, which suggests its potential role in the molecular therapy of brain cancer. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：76 / 82
页数：7
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