1, 25-Dihydroxyvitamin D3 suppresses cell cycle progression and thus growth of prostate cancer cells by inducing expression of limb bud and heart development (LBH)

被引：1
作者：
Du, Heng [1 ]
Zhuang, Jing [2 ]
Gui, Yaping [3 ]
Xu, Qiuyue [4 ]
Zhu, Li [5 ]
Gao, Le [1 ]
Jiang, Jie [5 ]
机构：
[1] Xinjiang Med Univ, Affiliated Hosp 2, Dept Urol, Xinjiang 860063, Peoples R China
[2] Peoples Hosp Xinjiang Uygur Autonomous Reg, Dept Nephrol, Urumqi 830000, Peoples R China
[3] Tongji Univ, Tongji Hosp Affiliated, Shanghai 200000, Peoples R China
[4] Nanjing Univ Chinese Med, Nanjing 210023, Jiangsu, Peoples R China
[5] Changchun Univ Tradit Chinese Med, Affiliated Hosp, Dept Urol, Changchun 130021, Jilin, Peoples R China
来源：
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2018年 / 17卷 / 09期
关键词：
Cell proliferation; Limb bud and heart development; Prostate cancer; 1; alpha; 25-Dihydroxyvitamin D3; INHIBITION; APOPTOSIS; GENE;
D O I：
10.4314/tjpr.v17i9.7
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Purpose: To investigate the function of limb bud and heart development (LBH) in 1 alpha, 25-dihydroxyvitamin D3 (1,25D)-mediated inhibitory effect on proliferation of prostate cancer cells. Methods: The inhibitory effect of 1,25D on growth and cell cycle progression of lymph node carcinoma of the prostate (LNCaP) cells was determined using cell counting kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry, while the expression levels of LBH in response to treatment with 1,25D were determined by quantitative reverse-transcription PCR (qRT-PCR) and western blottingting. The expression levels of LBH in cells were down/up regulated by transfection with siRNA or overexpression plasmids, and then cell growth and cell cycles were measured using the CCK8 assay, EdU assay, and flow cytometry. Finally, the growth inhibitory effect of 1,25D on LBH knockdown cells were determined using CCK-8 and EdU assays. Results: Treatment with 1,25D arrested LNCaP cells in G0/G1 phase of cell cycle, suppressed the growth of the cells and induced the expression of LBH. Overexpression/knockdown of LBH in LNCaP cells suppressed/promoted cell growth and accumulated/decreased cells in the G0/G1 phase. Moreover, knockdown of LBH reversed the inhibitory effect of 1,25D on cell proliferation of LNCaP cells. Conclusion: Inhibitory effect of 1,25D on cell cycle progression and cell proliferation might be via LBH.
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收藏
页码：1733 / 1738
页数：6
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