Selective isolation of Yersinia pestis from plague-infected fleas

被引:7
作者
Sarovich, Derek S. [1 ]
Colman, Rebecca E. [1 ]
Price, Erin P. [1 ]
Chung, Wai Kwan [1 ]
Lee, Judy [1 ]
Schupp, James M. [1 ]
Cobble, Kacy R. [1 ]
Busch, Joseph D. [1 ]
Alexander, James [2 ]
Keim, Paul [1 ]
Wagner, David M. [1 ]
机构
[1] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA
[2] Texas Dept State Hlth Serv, Canyon, TX USA
关键词
Plague; Yersinia pestis; Isolation; CIN agar; Selective; POLYMERASE-CHAIN-REACTION; PASTEURELLA-PESTIS; MOUSE INOCULATION; AGAR MEDIUM; CERATOPHYLLIDAE; SIPHONAPTERA; PULICIDAE; VECTOR; DYNAMICS; PCR;
D O I
10.1016/j.mimet.2010.03.019
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We evaluated Yersinia CIN agar for the isolation of Yersinia pestis from infected fleas. CIN media is effective for the differentiation of Y. pesos from flea commensal flora and is sufficiently inhibitory to other bacteria that typically outcompete Y. pestis after 48 h of growth using less selective media. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:95 / 97
页数:3
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