Spatially resolved transcriptomics and beyond

被引：369
作者：
Crosetto, Nicola
Bienko, Magda
van Oudenaarden, Alexander
机构：
[1] Royal Netherlands Acad Arts & Sci, Hubrecht Inst, KNAW, NL-3584 CT Utrecht, Netherlands
[2] Univ Med Ctr Utrecht, NL-3584 CT Utrecht, Netherlands
来源：
NATURE REVIEWS GENETICS | 2015年 / 16卷 / 01期
基金：
欧洲研究理事会;
关键词：
CELL RNA-SEQ; IN-SITU; GENOME-WIDE; INTRATUMOR HETEROGENEITY; OLIGONUCLEOTIDE PROBES; TUMOR HETEROGENEITY; IMAGE-ANALYSIS; SINGLE; RESOLUTION; EXPRESSION;
D O I：
10.1038/nrg3832
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Considerable progress in sequencing technologies makes it now possible to study the genomic and transcriptomic landscape of single cells. However, to better understand the complexity of multicellular organisms, we must devise ways to perform high-throughput measurements while preserving spatial information about the tissue context or subcellular localization of analysed nucleic acids. In this Innovation article, we summarize pioneering technologies that enable spatially resolved transcriptomics and discuss how these methods have the potential to extend beyond transcriptomics to encompass spatially resolved genomics, proteomics and possibly other omic disciplines.
引用
收藏
页码：57 / 66
页数：10
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