Multicolor single-particle reconstruction of protein complexes

被引：62
作者：
Sieben, Christian [1 ,2 ]
Banterle, Niccolo [3 ]
Douglass, Kyle M. [1 ]
Goenczy, Pierre [2 ,3 ]
Manley, Suliana [1 ,2 ]
机构：
[1] Ecole Polytech Fed Lausanne, Inst Phys, Lab Expt Biophys, Lausanne, Switzerland
[2] Univ Geneva, Swiss Natl Ctr Competence Res NCCR Chem Biol, Geneva, Switzerland
[3] Ecole Polytech Fed Lausanne, Swiss Inst Expt Canc Res, Sch Life Sci, Lausanne, Switzerland
来源：
NATURE METHODS | 2018年 / 15卷 / 10期
基金：
欧盟地平线“2020”; 欧洲研究理事会;
关键词：
SUPERRESOLUTION MICROSCOPY; ELECTRON-MICROSCOPY; CRYO-EM; ARCHITECTURE; CENTROSOMES; RESOLUTION; REVEALS; CELLS; PURIFICATION; ILLUMINATION;
D O I：
10.1038/s41592-018-0140-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.
引用
收藏
页码：777 / +
页数：6
相关论文
共 41 条
  • [1] Centriole Biogenesis: From Identifying the Characters to Understanding the Plot
    Banterle, Niccolo
    Gonczy, Pierre
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 33, 2017, 33 : 23 - 49
  • [2] Fourier ring correlation as a resolution criterion for super-resolution microscopy
    Banterle, Niccolo
    Khanh Huy Bui
    Lemke, Edward A.
    Beck, Martin
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2013, 183 (03) : 363 - 367
  • [3] Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging
    Bauer, Manuel
    Cubizolles, Fabien
    Schmidt, Alexander
    Nigg, Erich A.
    [J]. EMBO JOURNAL, 2016, 35 (19) : 2152 - 2166
  • [4] Snapshots of nuclear pore complexes in action captured by cryo-electron tomography
    Beck, Martin
    Lucic, Vladan
    Foerster, Friedrich
    Baumeister, Wolfgang
    Medalia, Ohad
    [J]. NATURE, 2007, 449 (7162) : 611 - 615
  • [5] STRUCTURAL AND CHEMICAL CHARACTERIZATION OF ISOLATED CENTROSOMES
    BORNENS, M
    PAINTRAND, M
    BERGES, J
    MARTY, MC
    KARSENTI, E
    [J]. CELL MOTILITY AND THE CYTOSKELETON, 1987, 8 (03): : 238 - 249
  • [6] The Centrosome in Cells and Organisms
    Bornens, Michel
    [J]. SCIENCE, 2012, 335 (6067) : 422 - 426
  • [7] Amplification and Purification of T4-Like Escherichia coli Phages for Phage Therapy: from Laboratory to Pilot Scale
    Bourdin, Gilles
    Schmitt, Bertrand
    Guy, Laure Marvin
    Germond, Jacques-Edouard
    Zuber, Sophie
    Michot, Lise
    Reuteler, Gloria
    Bruessow, Harald
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (04) : 1469 - 1476
  • [8] Structural Convergence between Cryo-EM and NMR Reveals Intersubunit Interactions Critical for HIV-1 Capsid Function
    Byeon, In-Ja L.
    Meng, Xin
    Jung, Jinwon
    Zhao, Gongpu
    Yang, Ruifeng
    Ahn, Jinwoo
    Shi, Jiong
    Concel, Jason
    Aiken, Christopher
    Zhang, Peijun
    Gronenborn, Angela M.
    [J]. CELL, 2009, 139 (04) : 780 - 790
  • [9] 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
    Campbell, Melody G.
    Veesler, David
    Cheng, Anchi
    Potter, Clinton S.
    Carragher, Bridget
    [J]. ELIFE, 2015, 4 : 1 - 22
  • [10] Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography
    Chang, Yi-Wei
    Kjaer, Andreas
    Ortega, Davi R.
    Kovacikova, Gabriela
    Sutherland, John A.
    Rettberg, Lee A.
    Taylor, Ronald K.
    Jensen, Grant J.
    [J]. NATURE MICROBIOLOGY, 2017, 2 (04):
12345