Nanoscale fabrication of a peptide layer in cell chip to detect effects of environmental toxins on HEK293 cells

被引：15

作者：

Kafi, Md. Abdul ^{[2
]}

Kim, Tae-Hyung ^{[1
]}

Yagati, Ajay Kumar ^{[2
]}

Kim, Hyuncheol ^{[1
]}

Choi, Jeong-Woo ^{[1
,2
]}

机构：

[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea

[2] Interdisciplinary Program Integrated Biotechnol, Seoul 121742, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 12期

关键词：

Cyclic voltammetry; Environmental toxicants; HEK-293; cells; RGD peptide; Scanning tunneling microscopy; Surface plasmon resonance; ASSAY; RGD;

D O I：

10.1007/s10529-010-0366-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cysteine-terminated C(RGD)(4) peptide film was fabricated on a gold electrode for improving the attachment of cells. The electrochemical signals of cyclic voltammogram from cells on a C(RGD)(4) deposited electrode was enhanced from 0.27 to 0.49 mu A compared to a bare electrode. The developed cell-based sensor determined the effect of bisphenol-A (BPA) and dichlorodiphenyltrichloroethane (DTT) on the viability of HEK-293 cells by detecting decrease of reduction peaks (1.12-0.15 mu A for BPA and 0.81-0.29 mu A for DDT) after the treatment of environmental chemicals. This developed system can be a powerful tool for the monitoring of environmental toxicants.

引用

页码：1797 / 1802

页数：6

共 14 条

[11] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

[12] RGD and other recognition sequences for integrins [J].

Ruoslahti, E .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :697-715

[13] Electrochemical monitoring of anticancer compounds on the human ovarian carcinoma cell line A2780 and its adriamycin- and cisplatin-resistant variants [J].

Woolley, DE ;

Tetlow, LC ;

Adlam, DJ ;

Gearey, D ;

Eden, RD ;

Ward, TH ;

Allen, TD .

EXPERIMENTAL CELL RESEARCH, 2002, 273 (01) :65-72

[14]

Yea CH, 2007, BIOCHIP J, V1, P219

← 1 2 →