Colorimetric determination of Timolol concentration based on localized surface plasmon resonance of silver nanoparticles

In this work, a rapid and simple colorimetric method based on the surface plasmon resonance of silver nanoparticles (AgNPs) was developed for the detection of the drug Timolol. The method used is based on the interaction of Timolol with the surface of the as-synthesized AgNPs, which promotes aggregation of the nanoparticles. This aggregation exploits the surface plasmon resonance through the electric dipole-dipole interaction and coupling among the agglomerated particles, hence bringing forth distinctive changes in the spectra as well as the color of colloidal silver. UV-vis spectrophotometery was used to monitor the changes of the localized surface plasmon resonance of AgNPs at wavelengths of 400 and 550 nm. The developed colorimetric sensor has a wide dynamic range of 1.0. x. 10(-7)M-1.0. x. 10(-3)M for detection of Timolol with a low detection limit of 1.2. x. 10(-6) M. The proposed method was successfully applied for the determination of Timolol concentration in ophthalmic eye-drop solution with a response time lower than 40 s.