Novel neutrophil-derived proteins in bronchoalveolar lavage fluid indicate an exaggerated inflammatory response in pediatric cystic fibrosis patients

Background: Airway inflammation in cystic fibrosis (CF) is exaggerated and characterized by neutrophilmediated tissue destruction, but its genesis and mechanisms remain poorly understood. To further define the pulmonary inflammatory response, we conducted a proteome-based screen of bronchoalveolar lavage fluid (BALF) collected from young children with and without CF experiencing endobronchial infection. Methods: We collected BALF samples from 45 children younger than 5 years and grouped them according to the presence of respiratory pathogens: >= 1 X 10(5) colony forming units (CFU)/mL BALF (18 and 12 samples with and without CF, respectively) and <1 X 10(5) CFU/mL (23 and 15 samples). BALF proteins were analyzed with SELDI-TOF mass spectrometry (MS) and H4 ProteinChips (R). Proteins were identified and characterized using trypsin digestion, tandem MS, Fourier transform ion cyclotron resonance MS, immunoblotting, and ELISA. Results: The SELDI-TOF MS BALF profiles contained 53 unique, reliably detected proteins. Peak intensities of 24 proteins differed significantly between the CF and non-CIF samples. They included the neutrophil proteins, alpha-defensin 1 and 2, S10OA8, SiOOA9, and S10OA12, as well as novel forms of S100A8 and S10OA12 with equivalent C-terminal deletions. Peak intensities of these neutrophil proteins and immunoreactive concentrations of selected examples were significantly higher in CF than non-CF samples. Conclusions: Small neutrophil-derived BALF proteins, including novel C-terminal truncated forms of S100A proteins, are easily detected with SELDI-TOF MS. Concentrations of these molecules are abnormally high in early CIF lung disease. The data provide new insights into CF lung disease and identify novel proteins strongly associated with CF airway inflammation. (C) 2007 American Association for Clinical Chemistry.