Transfer of PLRV resistance from Solanum verrucosum Schlechdt to potato (S-tuberosum L.) by protoplast electrofusion

被引：9

作者：

Carrasco, A ^{[1
]}

De Galarreta, JIR ^{[1
]}

Rico, A ^{[1
]}

Ritter, E ^{[1
]}

机构：

[1] NEIKER, CIMA, Vitoria 01080, Spain

来源：

POTATO RESEARCH | 2000年 / 43卷 / 01期

关键词：

somatic hybrids; potato leafroll virus; RAPD; Solanum wild species; pollen fertility;

D O I：

10.1007/BF02358511

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Somatic fusions between an accession of the diploid wild species Solanum verrucosum and a dihaploid S. tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were obtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.

引用

页码：31 / 42

页数：12

共 51 条

[1] TRANSFER OF RESISTANCE TO POTATO LEAF ROLL VIRUS FROM SOLANUM-BREVIDENS INTO SOLANUM-TUBEROSUM BY SOMATIC FUSION [J].