Isothermal sensitive detection of microRNA using an autonomous DNA machine recycling output as input

被引：13
作者：
Ogawa, Atsushi [1 ]
机构：
[1] Ehime Univ, Senior Res Fellow Ctr, Matsuyama, Ehime 7908577, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2010年 / 20卷 / 20期
关键词：
Nucleic acid detection; DNA machine; MicroRNA; Biosensor; Molecular beacon; ROLLING CIRCLE AMPLIFICATION; NUCLEIC-ACIDS; PARADIGM; RNA;
D O I：
10.1016/j.bmcl.2010.08.055
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
An autonomous DNA machine recycling the output as the input for isothermal, sensitive, and specific detection of miRNAs has been developed. This machine shows considerably high signal amplification efficiency (similar to 1000-fold) and thus a low detection limit (similar to 20 amol). The machine also shows high specificity, discriminating 50 amol of synthetic miRNA from 100-fold larger amounts of its family member and from 100 ng of unrelated total RNAs. Moreover, it is available for practically detecting natural miRNAs in total RNAs. (c) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6056 / 6060
页数：5
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  • [1] DNA-based machines
    Beissenhirtz, Moritz K.
    Willner, Itamar
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2006, 4 (18) : 3392 - 3401
  • [2] Revisiting the principles of microRNA target recognition and mode of action
    Brodersen, Peter
    Voinnet, Olivier
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (02) : 141 - 148
  • [3] Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes
    Cheglakov, Zoya
    Weizmann, Yossi
    Basnar, Bernhard
    Willner, Itamar
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (02) : 223 - 225
  • [4] Real-time quantification of microRNAs by stem-loop RT-PCR
    Chen, CF
    Ridzon, DA
    Broomer, AJ
    Zhou, ZH
    Lee, DH
    Nguyen, JT
    Barbisin, M
    Xu, NL
    Mahuvakar, VR
    Andersen, MR
    Lao, KQ
    Livak, KJ
    Guegler, KJ
    [J]. NUCLEIC ACIDS RESEARCH, 2005, 33 (20) : e179.1 - e179.9
  • [5] Cooperative Multicomponent Self-Assembly of Nucleic Acid Structures for the Activation of DNAzyme Cascades: A Paradigm for DNA Sensors and Aptasensors
    Elbaz, Johann
    Moshe, Michal
    Shlyahovsky, Bella
    Willner, Itamar
    [J]. CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (14) : 3411 - 3418
  • [6] Attomole microarray detection of MicroRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions
    Fang, Shiping
    Lee, Hye Jin
    Wark, Alastair W.
    Corn, Robert M.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (43) : 14044 - 14046
  • [7] miR-122, a paradigm for the role of microRNAs in the liver
    Girard, Muriel
    Jacquemin, Emmanuel
    Munnich, Arnold
    Lyonnet, Stanislas
    Henrion-Caude, Alexandra
    [J]. JOURNAL OF HEPATOLOGY, 2008, 48 (04) : 648 - 656
  • [8] Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction
    Guo, Qiuping
    Yang, Xiaohai
    Wang, Kemin
    Tan, Weihong
    Li, Wei
    Tang, Hongxing
    Li, Huimin
    [J]. NUCLEIC ACIDS RESEARCH, 2009, 37 (03)
  • [9] Identification of tissue-specific microRNAs from mouse
    Lagos-Quintana, M
    Rauhut, R
    Yalcin, A
    Meyer, J
    Lendeckel, W
    Tuschl, T
    [J]. CURRENT BIOLOGY, 2002, 12 (09) : 735 - 739
  • [10] Stand-Alone Rolling Circle Amplification Combined with Capillary Electrophoresis for Specific Detection of Small RNA
    Li, Ni
    Jablonowski, Carolyn
    Jin, Hailing
    Zhong, Wenwan
    [J]. ANALYTICAL CHEMISTRY, 2009, 81 (12) : 4906 - 4913
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