Transgenic mice carrying an Xist-containing YAC

被引：59
作者：
Heard, E
Kress, C
Mongelard, F
Courtier, B
Rougeulle, C
Ashworth, A
Vourch, C
Babinet, C
Avner, P
机构：
[1] INST PASTEUR, UNITE GENET MAMMIFERES, F-75724 PARIS 15, FRANCE
[2] FAC MED, INST ALBERT BONNIOT, EQUIPE DYOGEN, F-38706 LA TRONCHE, FRANCE
[3] INST CANC RES, CHESTER BEATTY LABS, CRC, CTR CELL & MOL BIOL, LONDON SW3 6JB, ENGLAND
来源：
HUMAN MOLECULAR GENETICS | 1996年 / 5卷 / 04期
基金：
英国惠康基金;
关键词：
D O I：
10.1093/hmg/5.4.441
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The initiation of X-chromosome inactivation in female mammals is controlled by a key locus, the X-inactivation centre (Xic). The Xist gene, which maps to the candidate region for Xic and is expressed exclusively from the inactive X chromosome, is thought to be an essential component of the Xic, To test whether sequences spanning several hundred kilobases and including Xist from the Xic region are capable of initiating inactivation, we have created a series of transgenic mice using a 460 kb yeast artificial chromosome (YAC). Analysis in these mice of the expression of Xist, of a LacZ reporter gene and of two genes in the region that are normally silent on the inactive X chromosome, suggests that essential sequences for Xist expression and X-inactivation may be absent in these transgenic animals.
引用
收藏
页码：441 / 450
页数：10
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