Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR

被引：45
作者：
Guilbaud, M
de Coppet, P
Bourion, F
Rachman, C
Prévost, H
Dousset, X
机构：
[1] ENITIAA, Lab Microbiol Alimentaire & Ind, F-44322 Nantes, France
[2] INRA, Unite Fonct Digest & Nutr Humaine, F-44026 Nantes, France
[3] Assoc ASEPT, Laval, PQ, Canada
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 04期
关键词：
D O I：
10.1128/AEM.71.4.2190-2194.2005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 X 10(2) CFU/cm(2).
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收藏
页码：2190 / 2194
页数：5
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