Silencing by blasting: combination of laser pulse induced stress waves and magnetophoresis for siRNA delivery

被引：6

作者：

Babincova, M. ^{[1
]}

Babincova, N. ^{[2
]}

Durdik, S. ^{[2
,3
]}

Bergemann, C. ^{[4
]}

Sourivong, P. ^{[5
]}

机构：

[1] Comenius Univ, Dept Nucl Phys & Biophys, Mlynska Dolina F1, Bratislava 84248, Slovakia

[2] Comenius Univ, Fac Med, Dept Surg Oncol, Spitalska 24, Bratislava 81372, Slovakia

[3] St Elizabeth Canc Inst, Heydukova 10, Bratislava 81250, Slovakia

[4] Chemicell GmbH, Eressburg Str 22-23, D-12103 Berlin, Germany

[5] Southwestern Reg Med Ctr, Dept Med Phys, Tulsa, OK USA

来源：

LASER PHYSICS LETTERS | 2016年 / 13卷 / 06期

关键词：

siRNA delivery; pulsed laser; stress waves; magnetofection; human carcinoma cells; GENE TRANSFECTION; MAGNETIC NANOPARTICLES; DOWN-REGULATION; CANCER CELLS; IN-VITRO; SYSTEM; MAGNETOFECTION; LIPOSOMES; FIELD; VIVO;

D O I：

10.1088/1612-2011/13/6/065601

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A new method is developed for efficient delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetic nanoparticle complex on the surface of cells with subsequent nanosecond laser pulse generating stress waves in transfection chamber, which is able to permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been shown using siRNA inducing cell apoptosis, combination of these two physical factors increased the efficiency of three different human carcinoma cells transfection to 93%, 89%, and 84%, for HeLa (cervical carcinoma), MCF-7 (breast carcinoma), and UCI-107 (ovarian carcinoma) cells, respectively. This new physical method of siRNA delivery may have therefore far reaching applications in biotechnology and functional genomics.

引用

页数：5

共 36 条

[1] Pressure and pressure gradient of laser-induced emergent stress waves using 0.07-mm-thick black rubber target [J].