Retinoic acid inhibits osteogenic differentiation of rat bone marrow stromal cells

被引：40
作者：
Wang, Anxun [1 ]
Ding, Xueqiang [1 ]
Sheng, Shihu [1 ]
Yao, Zhaoyou [1 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Oral & Maxillofacial Surg, Guangzhou 510080, Guangdong, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 375卷 / 03期
关键词：
retinoic acid; bone marrow stromal cells; osteogenesis; adipogenesis; differentiation; bone morphogenetic protein (BMP); signaling;
D O I：
10.1016/j.bbrc.2008.08.036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It has been well established that all-trans-retinoic acid (ATRA) influences bone metabolism when given in the treatment or prevention of cancer. However, the molecular mechanisms underlying this are unknown. In the present Study, we investigated the effect of ATRA on differentiation of rat bone marrow stromal cells (BMSCs). BMSCs were harvested from rats and induced to differentiate in the presence or absence of ATRA in either osteogenic (OM) or control medium (CM). BMSCs underwent osteogenic differentiation, showed alkaline phosphatase (ALP) activity, a high level of matrix mineralization, and expressed osteonectin when cultured in OM. Although ATRA induced ALP activity, it failed to induce matrix mineralization and osteonectin, decrease mineralization in OM, and induce lipid accumulation in BMSCs. Moreover, while ATRA induced the expression of BMP-RIA, both BMP-RII and Smad5 mRNA were induced by OM and ATRA. Thus, ATRA inhibited osteogenesis and promoted adipogenesis of BMSCs. BMP signaling cooperated with ATRA in the differentiation of BMSCs. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：435 / 439
页数：5
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