Effects of bisphenol A on the expression of cytochrome P450 aromatase (CYP19) in human fetal osteoblastic and granulosa cell-like cell lines

被引：27

作者：

Watanabe, Masatada ^{[1
]}

Ohno, Shuji ^{[1
]}

Nakajin, Shizuo ^{[1
]}

机构：

[1] Hoshi Univ, Sch Pharm & Pharmacal Sci, Dept Biochem, Shinagawa, Tokyo 1428501, Japan

来源：

TOXICOLOGY LETTERS | 2012年 / 210卷 / 01期

关键词：

Bisphenol A; Aromatase; Osteoblastic cells; Ovarian granulosa-like cells; TESTICULAR LEYDIG-CELLS; NUR77; GENE-EXPRESSION; ESTROGEN-RECEPTOR; ENDOCRINE DISRUPTION; THYROID-HORMONE; SV-HFO; ALPHA; STEROIDOGENESIS; ESTABLISHMENT; ASSOCIATION;

D O I：

10.1016/j.toxlet.2012.01.020

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The effects of bisphenol A (BPA), an endocrine disruptor. on aromatase (CYP19) expression in human osteoblastic (SV-HFO) and ovarian granulosa-like (KGN) cell lines were examined. CYP19 enzyme activity was suppressed in the presence of BPA in a dose-dependent fashion in both cell lines. CYP19 gene transcript expression, as well as activities of promoter I.4 in SV-HFO and promoter 11 in KGN, was down-regulated by SPA, suggesting that SPA affects CYP19 at the gene-expression level. These data and the previous finding that SPA induced the down-regulation of promoter I.1 activity within the human placental cell line suggest that there may be a conserved signaling pathway that down-regulates CYP19 expression in response to BPA in both cell lines. Additionally, differences between promoter I.4 and II suggest that there may be cell- and promoter-specific down-regulating mechanisms downstream from the actions of BPA. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：95 / 99

页数：5

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