Studies on the interaction between chromium(VI) and human serum albumin: Spectroscopic approach

被引：14

作者：

Zhang, Gen-Cheng ^{[1
,2
]}

Xu, Jie-Yan ^{[1
,2
]}

Wang, Yan-Qing ^{[1
,2
]}

机构：

[1] Yancheng Teachers Univ, Inst Appl Chem & Environ Engn, Yancheng City 224002, Jiangsu, Peoples R China

[2] Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng City 224002, Jiangsu, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2012年 / 86卷

关键词：

Chromium(VI); Human serum albumin; Fluorescence spectroscopy; Binding parameters; (VI)-INDUCED OXIDATIVE STRESS; HEXAVALENT CHROMIUM; BINDING; LIVER; WATER; RATS;

D O I：

10.1016/j.saa.2011.10.052

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The interaction between Cr2O72- and human serum albumin (HSA) was investigated using fluorescence, UV/vis, FT-IR, CD spectroscopy, and molecular modeling method. The experimental results showed that the fluorescence quenching of HSA by Cr2O72- is a result of the formation of HSA-chromium(VI) complex; static quenching was confirmed to result in the fluorescence quenching. The corresponding thermodynamic parameters showed that the process of binding Cr2O72- on HSA was a spontaneous molecular interaction procedure. Ionic, H-bonds and van der Waals interactions play a major role in stabilizing the complex. The Cr2O72- altered the environments of Trp and Tyr residues in HSA. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：381 / 386

页数：6

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