Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification

被引：11
作者：
Relloso, Maria S. [1 ]
Nievas, Jimena [1 ]
Fares Taie, Santiago [1 ]
Farquharson, Victoria [1 ]
Mujica, Maria T. [2 ]
Romano, Vanesa [1 ]
Zarate, Mariela S. [1 ]
Smayevsky, Jorgelina [1 ]
机构：
[1] Ctr Educ Med & Invest Clin Norberto Quirno CEMIC, Lab Bacteriol Micol & Parasitol, Buenos Aires, DF, Argentina
[2] Univ Buenos Aires, Inst Microbiol & Parasitol Med, CABA, IMPaM,UBA CONICET, RA-1053 Buenos Aires, DF, Argentina
来源：
REVISTA ARGENTINA DE MICROBIOLOGIA | 2015年 / 47卷 / 02期
关键词：
Yeast; MALDI-TOF MS; Identification; DESORPTION IONIZATION-TIME;
D O I：
10.1016/j.ram.2015.02.004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification. (C) 2014 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U. This is an open access article under the CC BY-NC-ND license.
引用
收藏
页码：103 / 107
页数：5
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