Tyrosinase biosensor based on zinc oxide nanorods

被引：17

作者：

Chen, L. Y. ^{[1
]}

Gu, B. X. ^{[2
]}

Zhu, G. P. ^{[2
]}

Wu, Y. F. ^{[1
]}

Liu, S. Q. ^{[1
]}

Xu, C. X. ^{[2
]}

机构：

[1] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Peoples R China

[2] Southeast Univ, Adv Photon Ctr, Sch Elect Sci & Engn, Nanjing 210096, Peoples R China

来源：

NANO | 2007年 / 2卷 / 05期

关键词：

ZnO nanorod; tyrosinase; biosensor; phenol; amperommetry;

D O I：

10.1142/S1793292007000647

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A phenol biosensor based on the skillful immobilization of tyrosinase on zinc oxide (ZnO) nanorods was proposed. The ZnO nanorods fabricated by a simple vapor-phase transport method possess a high aspect ratio, good electron communication, chemical purity, smooth and positive charged surface and are ready for immobilization of biochemicals with low isoelectric point (IEP). Electrochemical measurement and Scanning Electron Microscopic (SEM) analysis showed that the enzyme of tyrosinase with IEP 4.5 can be adsorbed on the surface of ZnO nanorods and kept its bioactivity of the oxidation of phenol to a large extent. This led us to develop phenol biosensor with good stability and reproducibility. The proposed method creates a new way to develop biosensors using nanostructured materials with high IEP.

引用

页码：281 / 284

页数：4

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