Single-Cell Quantification of a Highly Biocompatible Dinuclear Iridium(III) Complex for Photocatalytic Cancer Therapy

被引：69

作者：

Fan, Zhongxian ^{[1
]}

Rong, Yi ^{[2
]}

Sadhukhan, Tumpa ^{[3
]}

Liang, Shaoxia ^{[4
]}

Li, Wenqing ^{[1
]}

Yuan, Zhanxiang ^{[5
]}

Zhu, Zilin ^{[1
]}

Guo, Shunwen ^{[2
]}

Ji, Shaomin ^{[5
]}

Wang, Jinquan ^{[2
]}

Kushwaha, Rajesh ^{[6
]}

Banerjee, Samya ^{[6
]}

Raghavachari, Krishnan ^{[3
]}

Huang, Huaiyi ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Shenzhen Campus Sun Yat Sen Univ, Shenzhen 518107, Peoples R China

[2] Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Biotechnol Drug Candidates, Guangzhou 510006, Peoples R China

[3] Indiana Univ Bloomington, Dept Chem, Bloomington, IN 47405 USA

[4] PerkinElmer Inc, Guangzhou 510370, Peoples R China

[5] Guangdong Univ Technol, Light Ind & Chem Engn Coll, Guangzhou 510006, Peoples R China

[6] Indian Inst Technol BHU, Dept Chem, Varanasi 221005, UP, India

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2022年 / 61卷 / 23期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

Antitumor agents; Iridium Complexes; Photocatalysis; Photodynamic Therapy; Single-Cell ICP-MS; HALF-SANDWICH IRIDIUM(III); PHOTODYNAMIC THERAPY; POLYPYRIDYL COMPLEXES; PHOTOSENSITIZERS; MECHANISMS; PROBE;

D O I：

10.1002/anie.202202098

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Quantifying the content of metal-based anticancer drugs within single cancer cells remains a challenge. Here, we used single-cell inductively coupled plasma mass spectrometry to study the uptake and retention of mononuclear (Ir1) and dinuclear (Ir2) Ir-III photoredox catalysts. This method allowed rapid and precise quantification of the drug in individual cancer cells. Importantly, Ir2 showed a significant synergism but not an additive effect for NAD(P)H photocatalytic oxidation. The lysosome-targeting Ir2 showed low dark toxicity in vitro and in vivo. Ir2 exhibited high photocatalytic therapeutic efficiency at 525 nm with an excellent photo-index in vitro and in tumor-bearing mice model. Interestingly, the photocatalytic anticancer profile of the dinuclear Ir2 was much better than the mononuclear Ir1, indicating for the first time that dinuclear metal-based photocatalysts can be applied for photocatalytic anticancer treatment.

引用

页数：8

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