Reactions in double emulsions by flow-controlled coalescence of encapsulated drops

被引：82

作者：

Chen, Haosheng ^{[2
,3
]}

Zhao, Yuanjin ^{[3
]}

Li, Jiang ^{[1
,4
]}

Guo, Ming ^{[3
]}

Wan, Jiandi ^{[1
]}

Weitz, David A. ^{[3
]}

Stone, Howard A. ^{[1
]}

机构：

[1] Princeton Univ, Dept Mech & Aerosp Engn, Princeton, NJ 08544 USA

[2] Tsinghua Univ, State Key Lab Tribol, Beijing 100084, Peoples R China

[3] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[4] Univ Sci & Technol Beijing, Sch Mech Engn, Beijing 100083, Peoples R China

来源：

LAB ON A CHIP | 2011年 / 11卷 / 14期

关键词：

MONODISPERSE DOUBLE EMULSIONS; PROTEIN CRYSTALLIZATION; MICROFLUIDIC DEVICE; DROPLETS; BREAKUP; SHAPE; SIZE;

D O I：

10.1039/c1lc20265k

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate a microfluidic method to first generate double emulsion droplets containing two different inner drops, and to then control the internal coalescence of the encapsulated drops. The advantages of the core-coalescence method are illustrated by fabricating high viscosity particles and by demonstrating the dissolution of cell membranes.

引用

页码：2312 / 2315

页数：4

共 22 条

[1] Microfluidic sorting with high-speed single-layer membrane valves
Abate, Adam R.
Agresti, Jeremy J.
Weitz, David A.
[J]. APPLIED PHYSICS LETTERS, 2010, 96 (20)
[2] In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting
Bernath, K
Hai, MT
Mastrobattista, E
Griffiths, AD
Magdassi, S
Tawfik, DS
[J]. ANALYTICAL BIOCHEMISTRY, 2004, 325 (01) : 151 - 157
[3] Decompressing emulsion droplets favors coalescence
Bremond, Nicolas
Thiam, Abdou R.
Bibette, Jerome
[J]. PHYSICAL REVIEW LETTERS, 2008, 100 (02)
[4] Droplet microfluidic technology for single-cell high-throughput screening
Brouzes, Eric
Medkova, Martina
Savenelli, Neal
Marran, Dave
Twardowski, Mariusz
Hutchison, J. Brian
Rothberg, Jonathan M.
Link, Darren R.
Perrimon, Norbert
Samuels, Michael L.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (34) : 14195 - 14200
[5] Control of the rate of evaporation in protein crystallization by the 'microbatch under oil' method
Brumshtein, Boris
Greenblatt, Harry M.
Futerman, Anthony H.
Silman, Israel
Sussman, Joel L.
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2008, 41 : 969 - 971
[6] Separation-driven coalescence of droplets: an analytical criterion for the approach to contact
Lai, Ann
Bremond, Nicolas
Stone, Howard A.
[J]. JOURNAL OF FLUID MECHANICS, 2009, 632 : 97 - 107
[7] Flow induced coalescence of drops in a viscous fluid
Leal, LG
[J]. PHYSICS OF FLUIDS, 2004, 16 (06) : 1833 - 1851
[8] Shape-controlled production of biodegradable calcium alginate gel microparticles using a novel microfluidic device
Liu, Kan
Ding, Hui-Jiang
Liu, Jing
Chen, Yong
Zhao, Xing-Zhong
[J]. LANGMUIR, 2006, 22 (22) : 9453 - 9457
[9] Cell Tracing Dyes Significantly Change Single Cell Mechanics
Lulevich, Valentin
Shih, Yi-Ping
Lo, Su Hao
Liu, Gang-yu
[J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (18) : 6511 - 6519
[10] Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme
Mazutis, Linas
Baret, Jean-Christophe
Treacy, Patrick
Skhiri, Yousr
Araghi, Ali Fallah
Ryckelynck, Michael
Taly, Valerie
Griffiths, Andrew D.
[J]. LAB ON A CHIP, 2009, 9 (20) : 2902 - 2908

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