Spectral study of the interaction between enoxacin and calf thymus DNA

The spectroscopic behavior of enoxacin has been investigated in aqueous solutions. The absorption spectra of enoxacin DNA system were measured and interpreted. The binding of enoxacin to calf thymus DNA was considered in terms of Benesi-Hildebrand, Scott, Scatchard and Wolfe methods. The number of absorbing species presents in solution, the binding constant and the number of binding sites for each segment of DNA were determined. The modifications observed in the absorption spectra were interpreted in terms of the stacking of drug molecules, fixed by electrostatic interactions to the anionic groups from the biopolymer.