Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease

被引：38

作者：

Fulton, R. W. ^{[1
]}

d'Offay, J. M. ^{[1
]}

Landis, C. ^{[1
]}

Miles, D. G. ^{[2
]}

Smith, R. A. ^{[3
]}

Saliki, J. T. ^{[4
]}

Ridpath, J. F. ^{[5
]}

Confer, A. W. ^{[1
]}

Neill, J. D. ^{[5
]}

Eberle, R. ^{[1
]}

Clement, T. J. ^{[6
]}

Chase, C. C. L. ^{[6
]}

Burge, L. J. ^{[1
]}

Payton, M. E. ^{[7
]}

机构：

[1] Oklahoma State Univ, Dept Vet Pathobiol, Stillwater, OK 74078 USA

[2] Vet Res & Consulting Serv, Greeley, CO 80634 USA

[3] Vet Res & Consulting Serv, Stillwater, OK 74075 USA

[4] Univ Georgia, Athens Vet Diagnost Lab, Athens, GA 30602 USA

[5] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA

[6] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA

[7] Oklahoma State Univ, Dept Stat, Stillwater, OK 74078 USA

来源：

VACCINE | 2016年 / 34卷 / 30期

关键词：

Bovine respiratory viruses; MLV vaccines; Diagnostic virology; VIRAL-DIARRHEA-VIRUS; PERSISTENTLY INFECTED CATTLE; TIME RT-PCR; DIAGNOSTIC-TESTS; HERPESVIRUS-1; CORONAVIRUS; PATHOGENS; SUBTYPES; PREVALENCE; MORTALITY;

D O I：

10.1016/j.vaccine.2016.04.020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study investigated viruses in bovine respiratory disease (BRD) cases in feedlots, including bovine herpesvirus-1 (BoHV-1), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronaviruses (BoCV) and parainfluenza-3 virus (PI3V). Nasal swabs were collected from 114 cattle on initial BRD treatment. Processing included modified live virus (MLV) vaccination. Seven BRD necropsy cases were included for 121 total cases. Mean number of days on feed before first sample was 14.9 days. Swabs and tissue homogenates were tested by gel based PCR (G-PCR), quantitative-PCR (qPCR) and quantitative real time reverse transcriptase PCR (qRT-PCR) and viral culture. There were 87/114 (76.3%) swabs positive for at least one virus by at least one test. All necropsy cases were positive for at least one virus. Of 121 cases, positives included 18/121 (14.9%) BoHV-1; 19/121 (15.7%) BVDV; 76/121 (62.8%) BoCV; 11/121 (9.1%) BRSV; and 10/121 (8.3%) PI3V. For nasal swabs, G-PCR (5 viruses) detected 44/114 (38.6%); q-PCR and qRT-PCR (4 viruses) detected 81/114 (71.6%); and virus isolation detected 40/114 (35.1%). Most were positive for only one or two tests, but not all three tests. Necropsy cases had positives: 5/7 G-PCR, 5/7 q-PCR and qRT-PCR, and all were positive by cell culture. In some cases, G-PCR and both real time PCR were negative for BoHV-1, BVDV, and PI3V in samples positive by culture. PCR did not differentiate field from vaccines strains of BoHV-1, BVDV, and PI3V. However based on sequencing and analysis, field and vaccine strains of culture positive BoHV-1, BoCV, BVDV, and PI3V, 11/18 (61.1%) of BoHV-1 isolates, 6/17 (35.3%) BVDV isolates, and 1/10 (10.0%) PI3V identified as vaccine. BRSV was only identified by PCR testing. Interpretation of laboratory tests is appropriate as molecular based tests and virus isolation cannot separate field from vaccine strains. Additional testing using sequencing appears appropriate for identifying vaccine strains. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：3478 / 3492

页数：15

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