HPLC analysis of HI-6 dichloride and dimethanesulfonate - Antidotes against nerve agents and organophosphorus pesticides

被引：57
作者：
Jun, Daniel
Kuca, Kamil [1 ]
Stodulka, Petr
Koleckar, Vit
Dolezal, Bohuslav
Simon, Peter
Veverka, Miroslav
机构：
[1] Univ Def, Fac Mil Hlth Sci, Ctr Adv Studies, Hradec Kralove, Czech Republic
[2] Univ Def, Fac Mil Hlth Sci, Dept Toxicol, Hradec Kralove, Czech Republic
[3] Vakos XT AS, Prague, Czech Republic
[4] Slovak Tech Univ Bratislava, Fac Chem Technol & Food Technol, Dept Phys Chem, Bratislava, Slovakia
[5] Bel Novamann Int Ltd, Bratislava, Slovakia
来源：
ANALYTICAL LETTERS | 2007年 / 40卷 / 14期
关键词：
HPLC; HI-6; cholinesterase; reactivator; antidote; nerve agent; oxime;
D O I：
10.1080/00032710701588531
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Description of the simple HPLC method with UV detection for the identification of the acetylcholinesterase reactivator-HI-6 is described. Two salts of HI-6 (dichloride and dimethanesulfonate) were tested. Using this method 0.5 mu M LOD was determined. Our method could be used in future as standard method for the evaluation of HI-6 stability and purity.
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收藏
页码：2783 / 2787
页数：5
相关论文
共 15 条
[1]   Treatment of organophosphate intoxication using cholinesterase reactivators: Facts and fiction [J].
Bajgar, Jiri ;
Fusek, Josef ;
Kuca, Kamil ;
Bartosova, Lucie ;
Jun, Daniel .
MINI-REVIEWS IN MEDICINAL CHEMISTRY, 2007, 7 (05) :461-466
[2]   DETERMINATION OF SOME PYRIDINIUM ALDOXIME COMPOUNDS BY MEANS OF ION-PAIR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - APPLICATION IN BIOLOGICAL-MATERIAL [J].
BENSCHOP, HP ;
KONINGS, KAG ;
KOSSEN, SP ;
LIGTENSTEIN, DA .
JOURNAL OF CHROMATOGRAPHY, 1981, 225 (01) :107-114
[3]  
Hagedorn I, 1973, U. S. Patent, Patent No. [3,773,775, 3773775]
[4]  
JUN D, 2007, IN PRESS J CHROMATOG
[5]   In vitro comparison of eight acetylcholinesterase reactivators to reactivate VX inhibited ACHE [J].
Jun, Daniel ;
Bajgar, Jiri ;
Kuca, Kamil .
TOXICOLOGY LETTERS, 2006, 164 :S120-S120
[6]   Equipotent cholinesterase reactivation in vitro by the nerve agent antidotes HI 6 dichloride and HI 6 dimethanesulfonate [J].
Krummer, S ;
Thiermann, H ;
Worek, F ;
Eyer, P .
ARCHIVES OF TOXICOLOGY, 2002, 76 (10) :589-595
[7]   Comparison of the potency of newly developed and currently available oximes to reactivate nerve agent-inhibited acetylcholinesterase in vitro and in vivo [J].
Kuca, K ;
Bartosova, L ;
Kassa, J ;
Cabal, J ;
Bajgar, J ;
Kunesova, G ;
Jun, D .
CHEMICO-BIOLOGICAL INTERACTIONS, 2005, 157 :367-+
[8]   Structural requirements of acetylcholinesterase reactivators [J].
Kuca, K ;
Jun, D ;
Musilek, K .
MINI-REVIEWS IN MEDICINAL CHEMISTRY, 2006, 6 (03) :269-277
[9]   TLC determination of hydrophilicity parameter of some pyridinium aldoximes [J].
Laufer, Rudolf ;
Bathori, Maria ;
Csermely, Tamas ;
Petroianu, Georg ;
Kuca, Kamil ;
Toth, Noemi ;
Kalasz, Huba .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2007, 30 (13-16) :2337-2344
[10]   Entry of two new asymmetric bispyridinium oximes (K-27 and K-48) into the rat brain: comparison with obidoxime [J].
Lorke, D. E. ;
Hasan, M. Y. ;
Nurulain, S. M. ;
Sheen, R. ;
Kuca, K. ;
Petroianu, G. A. .
JOURNAL OF APPLIED TOXICOLOGY, 2007, 27 (05) :482-490
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