Detection of hepatitis E virus RNA in stools and serum by reverse transcription-PCR

被引：10
作者：
Turkoglu, S
Lazizi, Y
Meng, JH
Kordosi, A
Dubreuil, P
Crescenzo, B
Benjelloun, S
Nordmann, P
Pillot, J
机构：
[1] HOP ANTOINE BECLERE,SERV BACTERIOL VIROL,F-92140 CLAMART,FRANCE
[2] WHO,CTR REFERENCE & RES VIRAL HEPATITIS,UNITE IMMUNOL MICROBIENNE,PARIS,FRANCE
[3] INST PASTEUR,UNITE HEPATITES,CASABLANCA,MOROCCO
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 06期
关键词：
D O I：
10.1128/JCM.34.6.1568-1571.1996
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Stools and sera collected during an experimental hepatitis E virus (HEV) infection in monkeys and collected from humans with acute HEV infections during epidemic and sporadic cases were analyzed by reverse transcription-PCR. Two methods for RNA purification were compared. Proteinase K digestion and phenolchloroform extraction were more efficient than guanidinium isothiocyanate extraction in improving the sensitivity and specificity for the detection of HEV genomes.
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页码：1568 / 1571
页数：4
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