Comprehensive analyses of the annexin gene family in wheat

被引：52

作者：

Xu, Lei ^{[1
,4
]}

Tang, Yimiao ^{[1
]}

Gao, Shiqing ^{[1
]}

Su, Shichao ^{[5
]}

Hong, Lin ^{[1
]}

Wang, Weiwei ^{[1
]}

Fang, Zhaofeng ^{[1
]}

Li, Xueyin ^{[1
]}

Ma, Jinxiu ^{[1
]}

Quan, Wei ^{[1
]}

Sun, Hui ^{[1
]}

Li, Xia ^{[2
,3
]}

Wang, Yongbo ^{[1
]}

Liao, Xiangzheng ^{[1
]}

Gao, Jiangang ^{[1
]}

Zhang, Fengting ^{[1
]}

Li, Lei ^{[2
,3
]}

Zhao, Changping ^{[1
]}

机构：

[1] Beijing Acad Agr & Forestry Sci, Beijing Engn Res Ctr Hybrid Wheat, Municipal Key Lab Mol Genet Hybrid Wheat, Beijing 100097, Peoples R China

[2] Peking Univ, Sch Adv Agr Sci, Peking Tsinghua Ctr Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[3] Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China

[4] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China

[5] Hebei Normal Univ Sci & Technol, Coll Life Sci, Qinhuangdao 066600, Peoples R China

来源：

BMC GENOMICS | 2016年 / 17卷

基金：

美国国家科学基金会;

关键词：

Hybrid wheat; Annexin; Phylogenetic analysis; Calcium signaling; Cold induction; Thermosensitive genic male sterile (TGMS); ACTIN-DEPOLYMERIZING FACTOR; DIFFERENTIAL EXPRESSION; PLANT ANNEXINS; MEIOSIS I; CALCIUM; STRESS; ARABIDOPSIS; PROTEIN; GENOME; DROUGHT;

D O I：

10.1186/s12864-016-2750-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Annexins are an evolutionarily conserved multigene family of calcium-dependent phospholipid binding proteins that play important roles in stress resistance and plant development. They have been relatively well characterized in model plants Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa), but nothing has been reported in hexaploid bread wheat (Triticum aestivum) and barely (Hordeum vulgare), which are the two most economically important plants. Results: Based on available genomic and transcriptomic data, 25 and 11 putative annexin genes were found through in silico analysis in wheat and barley, respectively. Additionally, eight and 11 annexin genes were identified from the draft genome sequences of Triticum urartu and Aegilops tauschii, progenitor for the A and D genome of wheat, respectively. By phylogenetic analysis, annexins in these four species together with other monocots and eudicots were classified into six different orthologous groups. Pi values of each of Ann1-12 genes among T. aestivum, T. urartu, A. tauschii and H. vulgare species was very low, with the exception of Ann2 and Ann5 genes. Ann2 gene has been under positive selection, but Ann6 and Ann7 have been under purifying selection among the four species in their evolutionary histories. The nucleotide diversities of Ann1-12 genes in the four species were 0.52065, 0.59239, 0.60691 and 0.53421, respectively. No selective pressure was operated on annexin genes in the same species. Gene expression patterns obtained by real-time PCR and re-analyzing the public microarray data revealed differential temporal and spatial regulation of annexin genes in wheat under different abiotic stress conditions such as salinity, drought, cold and abscisic acid. Among those genes, TaAnn10 is specifically expressed in the anther but fails to be induced by low temperature in thermosensitive genic male sterile lines, suggesting that specific down-regulation of TaAnn10 is associated with conditional male sterility in wheat. Conclusions: This study analyzed the size and composition of the annexin gene family in wheat and barley, and investigated differential tissue-specific and stress responsive expression profiles of the gene family in wheat. These results provided significant information for understanding the diverse roles of plant annexins and opened a new avenue for functional studies of cold induced male sterility in wheat.

引用

页数：18

共 68 条

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