EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica

被引：19

作者：

Vandermies, Marie ^{[1
]}

Denies, Olivia ^{[1
]}

Nicaud, Jean-Marc ^{[2
]}

Fickers, Patrick ^{[1
]}

机构：

[1] Univ Liege Gembloux AgroBio Tech, TERRA Teaching & Res Ctr, Microbial Proc & Interact, Passage Deportes,2, B-5030 Gembloux, Belgium

[2] Univ Paris Saclay, AgroParisTech, INRA, Micalis Inst, Jouy En Josas, France

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 139卷

关键词：

Yarrowia lipolytica; Genome editing; EYK1; Catabolic selectable marker; EXPRESSION;

D O I：

10.1016/j.mimet.2017.05.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.

引用

页码：161 / 164

页数：4

共 11 条

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Carly F., 2017, APPL MICROB IN PRESS

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