Microbial Typing by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry: Do We Need Guidance for Data Interpretation?

被引：73

作者：

Spinali, Sebastien ^{[1
]}

van Belkum, Alex ^{[1
]}

Goering, Richard V. ^{[2
]}

Girard, Victoria ^{[1
]}

Welker, Martin ^{[1
]}

Van Nuenen, Marc ^{[3
]}

Pincus, David H. ^{[4
]}

Arsac, Maud ^{[5
]}

Durand, Geraldine ^{[1
]}

机构：

[1] BioMerieux SA, Microbiol Unit, R&D Microbiol, La Balme Les Grottes, France

[2] Creighton Univ, Med Ctr, Sch Med, Dept Med Microbiol & Immunol, Omaha, NE USA

[3] BioMerieux Inc, Microbiol Unit, Durham, NC USA

[4] BioMerieux Inc, St Louis, MO USA

[5] BioMerieux SA, Unit Innovat & Syst, Marcy Letoile, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 03期

关键词：

STREPTOCOCCUS-PNEUMONIAE; ESCHERICHIA-COLI; IDENTIFICATION; DIFFERENTIATION; PERFORMANCE; SYSTEM;

D O I：

10.1128/JCM.01635-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology has revolutionized species identification of bacteria, yeasts, and molds. However, beyond straightforward identification, the method has also been suggested to have the potential for subspecies-level or even type-level epidemiological analyses. This minireview explores MALDI-TOF MS-based typing, which has already been performed on many clinically relevant species. We discuss the limits of the method's resolution and we suggest interpretative criteria allowing valid comparison of strain-specific data. We conclude that guidelines for MALDI-TOF MS-based typing can be developed along the same lines as those used for the interpretation of data from pulsed-field gel electrophoresis (PFGE).

引用

页码：760 / 765

页数：6

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