Differential effects of excess high-fructose corn syrup on the DNA methylation of hippocampal neurotrophic factor in childhood and adolescence

被引：14

作者：

Kageyama, Itsuki ^{[1
,2
]}

Yamada, Hiroya ^{[3
]}

Munetsuna, Eiji ^{[4
]}

Yamazaki, Mirai ^{[5
]}

Ando, Yoshitaka ^{[1
]}

Mizuno, Genki ^{[2
]}

Fujii, Ryosuke ^{[2
]}

Nouchi, Yuki ^{[1
,2
]}

Wakasugi, Takuya ^{[1
]}

Sakakibara, Tomohide ^{[1
]}

Teshigawara, Atsushi ^{[1
]}

Ishikawa, Hiroaki ^{[1
]}

Shimono, Yohei ^{[4
]}

Suzuki, Koji ^{[2
]}

Hashimoto, Shuji ^{[3
]}

Ohashi, Koji ^{[1
]}

机构：

[1] Fujita Hlth Univ, Dept Informat Clin Med, Sch Med Sci, Toyoake, Aichi, Japan

[2] Fujita Hlth Univ, Dept Prevent Med Sci, Sch Med Sci, Toyoake, Aichi, Japan

[3] Fujita Hlth Univ, Dept Hyg, Sch Med, Toyoake, Aichi, Japan

[4] Fujita Hlth Univ, Dept Biochem, Sch Med, Toyoake, Aichi, Japan

[5] Kagawa Prefectural Univ Hlth Sci, Dept Med Technol, Takamatsu, Kagawa, Japan

来源：

PLOS ONE | 2022年 / 17卷 / 06期

基金：

日本学术振兴会;

关键词：

CIRCULATING MICRORNAS; SPATIAL MEMORY; RISK-FACTOR; BDNF; BRAIN; CONSUMPTION; EXPRESSION; NEUROGENESIS; INFLAMMATION; TRANSPORTER;

D O I：

10.1371/journal.pone.0270144

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Consumption of fructose-containing beverages such as high-fructose corn syrup (HFCS) is increasing, raising concerns about the negative effects of excessive fructose intake. A recent report indicated that excess HFCS intake impairs hippocampal function. In this study, we focused on neurotrophic factors (NFs) in the hippocampus from the viewpoint of epigenetics to clarify the adverse effects of fructose. We analyzed the effects of HFCS intake on hippocampal function in three age categories: childhood and adolescence (postnatal day (PD) 21-60), young adulthood (PD60-100), and late adulthood (PD100-140). For the experiments, male Sprague-Dawley rats were divided into three age categories, the control group was received distilled water and the HFCS group was received 20% HFCS solution for 40 days in each period. We analyzed mRNA and protein levels for qPCR and western blotting, respectively, of a hippocampal NF, brain-derived neurotrophic factor (Bdnf). HFCS consumption reduced hippocampal Bdnf mRNA and protein expressions in childhood and adolescence. Moreover, pyrosequencing assays revealed increased DNA methylation at the Bdnf promoter in childhood and adolescence. This Bdnf levels reduction may be due to hypermethylation of the promoter regions. It should be noted that this phenomenon was observed only in childhood and adolescence fructose consumption. Our results indicate that the sensitivity of the hippocampus to fructose may vary with age. This study provides insight into the adverse effects of excessive HFCS consumption on the hippocampus in children.

引用

页数：13

共 56 条

[1] Docosahexaenoic acid: A positive modulator of Akt signaling in neuronal survival [J].