Chondrocyte Cultivationin Hyaluronan-Tyramine Cross-Linked Hydrogel

被引：5
|
作者：
Kucera, Lukas [1 ,2 ]
Weinfurterova, Radka [1 ]
Dvorakova, Jana [1 ]
Kucera, Jan [1 ,3 ]
Pravda, Martin [1 ]
Foglarova, Marcela [1 ]
Svik, Karol [1 ]
Klein, Pavel [4 ]
Velebny, Vladimir [1 ]
Kubala, Lukas [5 ,6 ]
机构：
[1] Contipro Biotech Sro, Dolni Dobrouc, Czech Republic
[2] Masaryk Univ, Fac Sci, Dept Physiol & Immunol Anim, Inst Expt Biol, CS-61137 Brno, Czech Republic
[3] Charles Univ Prague, Dept Histol & Embryol, Med Fac Hradec Kralove, Hradec Kralove, Czech Republic
[4] Contipro Pharma As, Dolni Dobrouc, Czech Republic
[5] Acad Sci Czech Republ, Inst Biophys, CS-61265 Brno, Czech Republic
[6] St Annes Univ Hosp Brno, Ctr Biomol & Cellular Engn, Int Clin Res Ctr, Brno, Czech Republic
来源：
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS | 2015年 / 64卷 / 13期
关键词：
Biocompatibility; chondrogenesis; hyaluronate; scaffold; tyramine; ARTICULAR-CARTILAGE; GENE-EXPRESSION; IN-VITRO; MATRIX; DEFECTS; REPAIR; DIFFERENTIATION; CHONDROGENESIS; DEGRADATION; MODULATION;
D O I：
10.1080/00914037.2014.996715
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The application of chondrocyte-seeded hydrogels, such as tyramine-substituted hyaluronan, is a highly promising strategy for damaged cartilage treatments. The rheological characteristics of hyaluronan-tyramine derivative scaffold were determined together with the hydrogel swelling, the water uptake, and the morphological characterization. The chondrocytes seeded in hyaluronan-tyramine hydrogel were viable and expressed markers typical for differentiated chondrocytes with no signs of hypertrophy or extensive expression of matrix metalloproteinases. The lyophilized unseeded hyaluronan-tyramine scaffolds were also implantated into trochlea osteochondral defects in rabbits. In conclusion, the hyaluronan-tyramine derivative was proven to be advisable material as a scaffold for cartilage reconstitution. [GRAPHICS]
引用
收藏
页码：661 / 674
页数：14
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