Transcript profiling in plastid arginine tRNA-CCG gene knockout moss:: Construction of Physcomitrella patens plastid DNA microarray

被引：8

作者：

Nakamura, T ^{[1
]}

Sugiura, C ^{[1
]}

Kobayashi, Y ^{[1
]}

Sugita, M ^{[1
]}

机构：

[1] Nagoya Univ, Ctr Gene Res, Nagoya, Aichi 4648602, Japan

来源：

PLANT BIOLOGY | 2005年 / 7卷 / 03期

关键词：

chloroplast; Physcomitrella patens; transcript profiling; microarray;

D O I：

10.1055/s-2005-865620

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The moss Physcomitrella patens is a newly established model plant that is widely used for the characterization of gene function by targeted gene knockout or over-expression. The target gene disruption occurs in both the nuclear and chloroplast genomes. We applied DNA microarray technology to the P. patens plastid genome for large-scale analysis of transcripts. A microarray was constructed containing 108 DNA fragments to detect all annotated plastid genes. We analyzed the transcript profile in a knockout transformant for the arginine tRNA gene, trnR-CCG, and confirmed previous results that rbcL and psal transcripts accumulate in similar levels to wild-type moss, and accD transcript level is higher than those of wild-type moss. Additionally, the plastid DNA microarray revealed that most plastid genes were expressed at similar levels in wild-type and transformant mosses. This indicates that trnR-CCG is not essential for the expression of plastid genes.

引用

页码：258 / 265

页数：8

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