A microbial biosensor for 6-aminopenicillanic acid

被引：4
|
作者：
Galindo, E
Lagunas, F
Osuna, J
Soberón, X
García, JL
机构：
[1] Univ Nacl Autonoma Mexico, Inst Biotechnol, Dept Bioengn, Cuernavaca 62250, Morelos, Mexico
[2] Univ Nacl Autonoma Mexico, Inst Biotechnol, Dept Mol Recognit & Biostruct, Cuernavaca 62250, Morelos, Mexico
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1998年 / 23卷 / 05期
关键词：
D O I：
10.1016/S0141-0229(98)00057-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A protein-engineered beta-lactamase (N132 D with enhanced selectivity to 6-APA was used to construct a whole-cell immersible biosensor for 6-APA based on the immobilization of beta-lactamase-rich cells of Escherichia coli on a flat pH electrode. The response time was between 3.5-7 min. The sensitivity for penicillin was two to five fold lower to that for 6-APA. Selectivity of 6-APA was maximum at pH 7.6 using a 0.05 M phosphate buffer. 6-APA in concentrations 1mg to ml(-1) could be detected. At least 70 analyses could be performed over II days with no loss of sensitivity. (C) 1998 Elsevier Science Inc.
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页码：331 / 334
页数：4
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