Distribution and activity levels of matrix metalloproteinase 2 and 9 in canine and feline osteosarcoma

被引：2

作者：

Gebhard, Christiane ^{[1
]}

Fuchs-Baumgartinger, Andrea ^{[2
]}

Razzazi-Fazeli, Ebrahim ^{[3
]}

Miller, Ingrid ^{[4
]}

Walter, Ingrid ^{[1
]}

机构：

[1] Univ Vet Med, Inst Anat Histol & Embryol, Veterinaerpl 1, A-1210 Vienna, Austria

[2] Univ Vet Med, Inst Pathol & Forens Vet Med, Veterinaerpl 1, A-1210 Vienna, Austria

[3] Univ Vet Med, VetCore Facil Res, Veterinaerpl 1, A-1210 Vienna, Austria

[4] Univ Vet Med, Inst Med Biochem, Veterinaerpl 1, A-1210 Vienna, Austria

来源：

CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE | 2016年 / 80卷 / 01期

基金：

奥地利科学基金会;

关键词：

EXTRACELLULAR-MATRIX; MMP-9; MATRIX-METALLOPROTEINASE-9; TISSUE; EXPRESSION; INVASION; COLLAGEN; TUMORS; INHIBITION; CLEAVAGE;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Overexpression of matrix metalloproteinases (MMPs) has been associated with increased tumor aggressiveness and metastasis dissemination. We investigated whether the contrasting metastatic behavior of feline and canine osteosarcoma is related to levels and activities of MMP2 and MMP9. Zymography and immunohistochemistry were used to determine expression levels of MMP2 and MMP9 in canine and feline osteosarcoma. Using immunohistochemistry, increased MMP9 levels were identified in most canine osteosarcomas, whereas cat samples more often displayed moderate levels. High levels of pro-MMP9, pro-MMP2, and active MMP2 were detected by gelatin zymography in both species, with significantly higher values for active MMP2 in canine osteosarcoma. These findings indicate that MMP2 is probably involved in canine and feline osteosarcoma and their expression and activity could be associated with the different metastatic behavior of canine and feline osteosarcoma.

引用

页码：66 / 73

页数：8

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