Haplotype analysis of the genes encoding glutamine synthetase plastic isoforms and their association with nitrogen-use- and yield-related traits in bread wheat

被引：63

作者：

Li, Xin-Peng ^{[1
]}

Zhao, Xue-Qiang ^{[1
]}

He, Xue ^{[1
]}

Zhao, Guang-Yao ^{[2
]}

Li, Bin ^{[1
]}

Liu, Dong-Cheng ^{[1
]}

Zhang, Ai-Min ^{[1
]}

Zhang, Xue-Yong ^{[2
]}

Tong, Yi-Ping ^{[1
]}

Li, Zhen-Sheng ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

来源：

NEW PHYTOLOGIST | 2011年 / 189卷 / 02期

基金：

中国国家自然科学基金;

关键词：

agronomic trait; association analysis; glutamine synthetase; haplotype; nitrogen-use efficiency; wheat; CELLULAR-LOCALIZATION; AMMONIUM ASSIMILATION; RICE; EXPRESSION; CHLOROPLAST; QTL; IDENTIFICATION; SYNTHASE; PROTEIN; INTRON;

D O I：

10.1111/j.1469-8137.2010.03490.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P>Glutamine synthetase (GS) plays a key role in the growth, nitrogen (N) use and yield potential of cereal crops. Investigating the haplotype variation of GS genes and its association with agronomic traits may provide useful information for improving wheat N-use efficiency and yield. We isolated the promoter and coding region sequences of the plastic glutamine synthetase isoform (GS2) genes located on chromosomes 2A, 2B and 2D in bread wheat. By analyzing nucleotide sequence variations of the coding region, two, six and two haplotypes were distinguished for TaGS2-A1 (a and b), TaGS2-B1 (a-f) and TaGS2-D1 (a and b), respectively. By analyzing the frequency data of different haplotypes and their association with N use and agronomic traits, four major and favorable TaGS2 haplotypes (A1b, B1a, B1b, D1a) were revealed. These favorable haplotypes may confer better seedling growth, better agronomic performance, and improved N uptake during vegetative growth or grain N concentration. Our data suggest that certain TaGS2 haplotypes may be valuable in breeding wheat varieties with improved agronomic performance and N-use efficiency.

引用

页码：449 / 458

页数：10

共 44 条

[1] Mapping QTLs for nitrogen uptake in relation to the early growth of wheat (Triticum aestivum L.) [J].

An, Diaoguo ;

Su, Junying ;

Liu, Quanyou ;

Zhu, Yongguan ;

Tong, Yiping ;

Li, Junming ;

Jing, Ruilian ;

Li, Bin ;

Li, Zhensheng .

PLANT AND SOIL, 2006, 284 (1-2) :73-84

[2] Recent amplification and impact of MITEs on the genome of grapevine (Vitis vinifera L.) [J].

Benjak, Andrej ;

Boue, Stephanie ;

Forneck, Astrid ;

Casacuberta, Josep M. .

GENOME BIOLOGY AND EVOLUTION, 2009, 1 :75-84

[3] Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.) [J].

Bernard, Stephanie M. ;

Moller, Anders Laurell Blom ;

Dionisio, Giuseppe ;

Kichey, Thomas ;

Jahn, Thomas P. ;

Dubois, Frederic ;

Baudo, Marcela ;

Lopes, Marta S. ;

Terce-Laforgue, Therese ;

Foyer, Christine H. ;

Parry, Martin A. J. ;

Forde, Brian G. ;

Araus, Jose Luis ;

Hirel, Bertrand ;

Schjoerring, Jan K. ;

Habash, Dimah Z. .

PLANT MOLECULAR BIOLOGY, 2008, 67 (1-2) :89-105

[4] Glutamine synthetase in the phloem plays a major role in controlling proline production [J].

Brugière, N ;

Dubois, F ;

Limami, AM ;

Lelandais, M ;

Roux, Y ;

Sangwan, RS ;

Hirel, B .

PLANT CELL, 1999, 11 (10) :1995-2011

[5] Ammonium assimilation and amino acid metabolism in conifers [J].

Canovas, Francisco M. ;

Avila, Concepcion ;

Canton, Francisco R. ;

Canas, Rafael A. ;

de la Torre, Fernando .

JOURNAL OF EXPERIMENTAL BOTANY, 2007, 58 (09) :2307-2318

[6]

Casacuberta JM, 2003, GENE, V311, P1, DOI 10.1016/S0378-1119(03)00557-2

[7]

Coruzzi Gloria M, 2003, Arabidopsis Book, V2, pe0010, DOI 10.1199/tab.0010

[8] CELL-SPECIFIC EXPRESSION IN TRANSGENIC PLANTS REVEALS NONOVERLAPPING ROLES FOR CHLOROPLAST AND CYTOSOLIC GLUTAMINE-SYNTHETASE [J].

EDWARDS, JW ;

WALKER, EL ;

CORUZZI, GM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) :3459-3463

[9] HIGH-LEVEL TUBER EXPRESSION AND SUCROSE INDUCIBILITY OF A POTATO SUS4 SUCROSE SYNTHASE GENE REQUIRE 5'-FLANKING AND 3'-FLANKING SEQUENCES AND THE LEADER INTRON [J].

FU, HY ;

KIM, SY ;

PARK, WD .

PLANT CELL, 1995, 7 (09) :1387-1394

[10] Developmental regulation of a cytosolic ascorbate peroxidase gene from tomato plants [J].

Gadea, J ;

Conejero, V ;

Vera, P .

MOLECULAR AND GENERAL GENETICS, 1999, 262 (02) :212-219

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