Polyphosphatase PPN1 of Saccharomyces cerevisiae: Switching of Exopolyphosphatase and Endopolyphosphatase Activities

被引：29

作者：

Andreeva, Nadezhda ^{[1
]}

Trilisenko, Ludmila ^{[1
]}

Eldarov, Mikhail ^{[2
]}

Kulakovskaya, Tatiana ^{[1
]}

机构：

[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia

[2] Russian Acad Sci, Ctr Bioengn, Moscow 117312, Russia

来源：

PLOS ONE | 2015年 / 10卷 / 03期

关键词：

INORGANIC POLYPHOSPHATE; PURIFICATION; PHOSPHATE; GROWTH; KINASE; OVEREXPRESSION; MINERALIZATION; COMPARTMENTS; CYTOSOL; PPX1;

D O I：

10.1371/journal.pone.0119594

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The polyphosphatase PPN1 of Saccharomyces cerevisiae shows an exopolyphosphatase activity splitting phosphate from chain end and an endopolyphosphatase activity fragmenting high molecular inorganic polyphosphates into shorter polymers. We revealed the compounds switching these activities of PPN1. Phosphate release and fragmentation of high molecular polyphosphate prevailed in the presence of Co2+ and Mg2+, respectively. Phosphate release and polyphosphate chain shortening in the presence of Co2+ were inhibited by ADP but not affected by ATP and arginine. The polyphosphate chain shortening in the presence of Mg2+ was activated by ADP and arginine but inhibited by ATP.

引用

页数：11

共 41 条

[11] Polyphosphate-mediated modulation of Campylobacter jejuni biofilm growth and stability [J].

Drozd, Mary ;

Chandrashekhar, Kshipra ;

Rajashekara, Gireesh .

VIRULENCE, 2014, 5 (06) :680-690

[12] Polyphosphates and exopolyphosphatase activities in the yeast Saccharomyces cerevisiae under overexpression of homologous and heterologous PPN1 genes [J].

Eldarov, M. A. ;

Baranov, M. V. ;

Dumina, M. V. ;

Shgun, A. A. ;

Andreeva, N. A. ;

Trilisenko, L. V. ;

Kulakovskaya, T. V. ;

Ryasanova, L. P. ;

Kulaev, I. S. .

BIOCHEMISTRY-MOSCOW, 2013, 78 (08) :946-953

[13] EFFECTS OF GROWTH-STATE AND AMINES ON CYTOPLASMIC AND VACUOLAR PH, PHOSPHATE AND POLYPHOSPHATE LEVELS IN SACCHAROMYCES-CEREVISIAE - A P-31-NUCLEAR MAGNETIC-RESONANCE STUDY [J].

GREENFIELD, NJ ;

HUSSAIN, M ;

LENARD, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 926 (03) :205-213

[14] Catalytic Core of a Membrane-Associated Eukaryotic Polyphosphate Polymerase [J].

Hothorn, Michael ;

Neumann, Heinz ;

Lenherr, Esther D. ;

Wehner, Mark ;

Rybin, Vladimir ;

Hassa, Paul O. ;

Uttenweiler, Andreas ;

Reinhardt, Monique ;

Schmidt, Andrea ;

Seiler, Jeanette ;

Ladurner, Andreas G. ;

Herrmann, Christian ;

Scheffzek, Klaus ;

Mayer, Andreas .

SCIENCE, 2009, 324 (5926) :513-516

[15] PURIFICATION OF POLYPHOSPHATE AND ATP GLUCOSE PHOSPHOTRANSFERASE FROM MYCOBACTERIUM-TUBERCULOSIS H37RA - EVIDENCE THAT POLY(P) AND ATP GLUCOKINASE ACTIVITIES ARE CATALYZED BY THE SAME ENZYME [J].

HSIEH, PC ;

SHENOY, BC ;

JENTOFT, JE ;

PHILLIPS, NFB .

PROTEIN EXPRESSION AND PURIFICATION, 1993, 4 (01) :76-84

[16]

Kulakovskaya T. V., 1997, BIOCHEMISTRY-MOSCOW, V62, P1180

[17] Role of inorganic polyphosphate in promoting ribosomal, protein degradation by the ion protease in E-coli [J].

Kuroda, A ;

Nomura, K ;

Ohtomo, R ;

Kato, J ;

Ikeda, T ;

Takiguchi, N ;

Ohtake, H ;

Kornberg, A .

SCIENCE, 2001, 293 (5530) :705-708

[18] Properties of Partially Purified Endopolyphosphatase of the Yeast Saccharomyces cerevisiae [J].

Lichko, L. P. ;

Kulakovskaya, T. V. ;

Kulaev, I. S. .

BIOCHEMISTRY-MOSCOW, 2010, 75 (11) :1404-1407

[19] Inorganic polyphosphates and exopolyphosphatases in cell compartments of the yeast Saccharomyces cerevisiae under inactivation of PPX1 and PPN1 genes [J].

Lichko, Lidiya ;

Kulakovskaya, Tatyana ;

Pestov, Nikolai ;

Kulaev, Igor .

BIOSCIENCE REPORTS, 2006, 26 (01) :45-54

[20] Identification of an Evolutionarily Conserved Family of Inorganic Polyphosphate Endopolyphosphatases [J].

Lonetti, Annalisa ;

Szijgyarto, Zsolt ;

Bosch, Daniel ;

Loss, Omar ;

Azevedo, Cristina ;

Saiardi, Adolfo .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (37) :31966-31974

← 1 2 3 4 5 →