Diphenyl diselenide (PhSe)2 inhibits biofilm formation by Candida albicans, increasing both ROS production and membrane permeability

Project: The opportunistic fungal Candida albicans can produce superficial and systemic infections in immunocompromised patients. An essential stage to both colonization and virulence by C. albicans is the transition from budding yeast form to filamentous form, producing biofilms. Procedure: In this work, we studied the effect of the organochalcogenide compound (PhSe)(2) on both cell growth and biofilm formation by C. albicans. Results: (PhSe)(2) inhibited both growth and biofilm formation by C. albicans. The inhibitory effects of (PhSe)2 depended on the cell density and (PhSe)(2) concentration. We have also observed that (PhSe)(2) stimulated ROS production (67%) and increased cell membrane permeability (2.94-fold) in C. albicans. In addition, (PhSe)(2) caused a marked decrease in proteinase activity (6.8-fold) in relation to non-treated group. Conclusions: (PhSe)(2) decreased both cell growth and biofilm development, decreasing the release of extracellular proteinases, which is an important facet of C. albicans pathogenicity. The toxicity of (PhSe)(2) towards C. albicans can be associated with an increase in ROS production, which can increase cell permeability. The permanent.damage to the cell membranes can culminate in cell death. (C) 2014 Elsevier GmbH. All rights reserved.