Pseudomonas aeruginosa injects type III effector ExoS into epithelial cells through the function of type IV pili

被引：19

作者：

Hayashi, Naoki ^{[1
]}

Nishizawa, Hideyuki ^{[1
]}

Kitao, Seiya ^{[1
]}

Deguchi, Sakurako ^{[1
]}

Nakamura, Takano ^{[1
]}

Fujimoto, Akiyo ^{[1
]}

Shikata, Mototsugu ^{[1
]}

Gotoh, Naomasa ^{[1
]}

机构：

[1] Kyoto Pharmaceut Univ, Dept Microbiol & Infect Control Sci, Kyoto 6078414, Japan

来源：

FEBS LETTERS | 2015年 / 589卷 / 08期

基金：

日本学术振兴会;

关键词：

Type IV pili; Type III secretion system; ExoS; Bacterial translocation; Epithelial cell; Pseudomonas aeruginosa; TWITCHING MOTILITY; INFECTION; COLONIZATION; VIRULENCE; MEMBRANE; BINDING; GENE; TRANSLOCATION; PENETRATION; JUNCTIONS;

D O I：

10.1016/j.febslet.2015.02.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translocation of Pseudomonas aeruginosa through epithelial tissues can cause sepsis. Here, we examined whether P. aeruginosa penetrates epithelial cell layers using type IV pili (TFP). Deletion of TFP (pilA) did not affect association with Caco-2 cells, although it decreased penetration through, and disruption of, Caco-2 cell monolayers. We found that TFP are necessary for injection of the type III effector ExoS, which impairs defense against P. aeruginosa penetration, into host cells. Deletion of pilA attenuated oral infection in silkworms. We conclude that P. aeruginosa injects ExoS into cells through the function of TFP, enabling penetration of epithelial barriers. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：890 / 896

页数：7

共 45 条

[1] Role of the Corneal Epithelial Basement Membrane in Ocular Defense against Pseudomonas aeruginosa [J].