Different surface charge of colistin-susceptible and -resistant Acinetobacter baumannii cells measured with zeta potential as a function of growth phase and colistin treatment

被引:98
作者
Soon, Rachel L. [1 ]
Nation, Roger L. [1 ]
Cockram, Stewart [1 ]
Moffatt, Jennifer H. [2 ]
Harper, Marina [2 ,3 ]
Adler, Ben [2 ,3 ]
Boyce, John D. [2 ,3 ]
Larson, Ian [1 ]
Li, Jian [1 ]
机构
[1] Monash Univ, Facil Antiinfect Drug Dev & Innovat Drug Delivery, Monash Inst Pharmaceut Sci, Parkville, Vic 3052, Australia
[2] Monash Univ, Dept Microbiol, Parkville, Vic 3052, Australia
[3] Monash Univ, Australian Res Council Ctr Excellence Struct & Fu, Parkville, Vic 3052, Australia
基金
英国医学研究理事会; 美国国家卫生研究院;
关键词
physicochemical properties; Gram-negative; polymyxin; OUTER-MEMBRANE PERMEABILITY; ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; POLYMYXIN-B; ANTIMICROBIAL PEPTIDES; YERSINIA-PESTIS; SOFT PARTICLES; BAD BUGS; ADHESION; 4-AMINO-4-DEOXY-L-ARABINOSE;
D O I
10.1093/jac/dkq422
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Electrostatic forces mediate the initial interaction between cationic colistin and Gram-negative bacterial cells. Lipopolysaccharide (LPS) loss mediates colistin resistance in some A. baumannii strains. Our aim was to determine the surface charge of colistin-susceptible and -resistant A. baumannii as a function of growth phase and in response to polymyxin treatment. The zeta potential of A. baumannii ATCC 19606 and 10 clinical multidrug-resistant strains (MICs 0.5-2 mg/L) was assessed. Colistin-resistant derivatives (MIC > 128 mg/L) of wild-type strains were selected in the presence of 10 mg/L colistin, including the LPS-deficient lpxA mutant, ATCC 19606R. To determine the contribution of LPS to surface charge, two complemented ATCC 19606R derivatives were examined, namely ATCC 19606R + lpxA (containing an intact lpxA gene) and ATCC 19606R + V (containing empty vector). Investigations were conducted as a function of growth phase and polymyxin treatment (1, 4 and 8 mg/L). Wild-type cells exhibited a greater negative charge (-60.5 +/- 2.36 to -26.2 +/- 2.56 mV) thancolistin-resistant cells (-49.2 +/- 3.09 to -19.1 +/- 2.80 mV) at mid-log phase (ANOVA, P < 0.05). Opposing growth-phase trends were observed for both phenotypes: wild-type cells displayed reduced negative charge and colistin-resistant cells displayed increased negative charge at stationary compared with mid-logarithmic phase. Polymyxin exposure resulted in a concentration-dependent increase in zeta potential. Examination of ATCC 19606R and complemented strains supported the importance of LPS in determining surface charge, suggesting a potential mechanism of colistin resistance. Zeta potential differences between A. baumannii phenotypes probably reflect compositional outer-membrane variations that impact the electrostatic component of colistin activity.
引用
收藏
页码:126 / 133
页数:8
相关论文
共 55 条
[1]   Escherichia coli Cell Surface Perturbation and Disruption Induced by Antimicrobial Peptides BP100 and pepR [J].
Alves, Carla S. ;
Melo, Manuel N. ;
Franquelim, Henri G. ;
Ferre, Rafael ;
Planas, Marta ;
Feliu, Lidia ;
Bardaji, Eduard ;
Kowalczyk, Wioleta ;
Andreu, David ;
Santos, Nuno C. ;
Fernandes, Miguel X. ;
Castanho, Miguel A. R. B. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (36) :27536-27544
[2]   Alterations in Two-Component Regulatory Systems of phoPQ and pmrAB Are Associated with Polymyxin B Resistance in Clinical Isolates of Pseudomonas aeruginosa [J].
Barrow, Kaddy ;
Kwon, Dong H. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (12) :5150-5154
[3]   Yersinia pseudotuberculosis and Yersinia pestis are more resistant to bactericidal cationic peptides than Yersinia enterocolitica [J].
Bengoechea, JA ;
Lindner, B ;
Seydel, U ;
Díaz, R ;
Moriyón, I .
MICROBIOLOGY-UK, 1998, 144 :1509-1515
[4]   Correlating Transport Behavior with Cell Properties for Eight Porcine Escherichia coli Isolates [J].
Bolster, Carl H. ;
Cook, Kimberly L. ;
Marcus, Ian M. ;
Haznedaroglu, Berat Z. ;
Walker, Sharon L. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2010, 44 (13) :5008-5014
[5]   Surface of lactic acid bacteria: Relationships between chemical composition and physicochemical properties [J].
Boonaert, CJP ;
Rouxhet, PG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (06) :2548-2554
[6]   Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America [J].
Boucher, Helen W. ;
Talbot, George H. ;
Bradley, John S. ;
Edwards, John E., Jr. ;
Gilbert, David ;
Rice, Louis B. ;
Scheld, Michael ;
Spellberg, Brad ;
Bartlett, John .
CLINICAL INFECTIOUS DISEASES, 2009, 48 (01) :1-12
[7]   Growth-phase regulation of lipopolysaccharide O-antigen chain length influences serum resistance in serovars of Salmonella [J].
Bravo, Denlsse ;
Silva, Cecllia ;
Carter, Javier A. ;
Hoare, Anllei ;
Alvarez, Serglo A. ;
Blondel, Carlos J. ;
Zaldivar, Mercedes ;
Valvano, Miguel A. ;
Contreras, Ines .
JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (08) :938-946
[8]   Effects of cell surface damage on surface properties and adhesion of Pseudomonas aeruginosa [J].
Bruinsma, GM ;
Rustema-Abbing, M ;
van der Mei, HC ;
Busscher, HJ .
JOURNAL OF MICROBIOLOGICAL METHODS, 2001, 45 (02) :95-101
[9]   Relevance of electrokinetic theory for "soft" particles to bacterial cells: Implications for bacterial adhesion [J].
de Kerchove, AJ ;
Elimelech, M .
LANGMUIR, 2005, 21 (14) :6462-6472
[10]   Effect of growth time on the surface and adhesion properties of Lactobacillus rhamnosus GG [J].
Deepika, G. ;
Green, R. J. ;
Frazier, R. A. ;
Charalampopoulos, D. .
JOURNAL OF APPLIED MICROBIOLOGY, 2009, 107 (04) :1230-1240