Screening of lactic acid bacteria producing uricase and stability assessment in simulated gastrointestinal conditions

被引：0

作者：

Handayani, I ^{[1
]}

Utami, T. ^{[2
]}

Hidayat, C. ^{[2
]}

Rahayu, E. S. ^{[2
]}

机构：

[1] Univ Jenderal Soedirman, Fac Agr, Dept Agr Technol, Jl Dr Soeparno, Karangwangkal Purwokerto 53123, Central Java, Indonesia

[2] Gadjah Mada Univ, Fac Agr Technol, Dept Food & Agr Prod Technol, Jl Flora 1 Bulaksumur, Yogyakarta 55281, Indonesia

来源：

INTERNATIONAL FOOD RESEARCH JOURNAL | 2018年 / 25卷 / 04期

关键词：

Lactic acid bacteria; Stability; Gastrointestinal system; Uricase; THERMOSTABLE URICASE; BETA-GALACTOSIDASE; URATE OXIDASE; PURIFICATION; DEGRADATION; ENZYME;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Thirteen probiotic lactic acid bacterial strains were screened for extracellular and intracellular uricase production. Results showed that all the strains could produce uricase with three strains, namely Lactobacillus sp.OL-5, Lactobacillus plantarum Mut-7, and Lactobacillus plantarum Dad-13, producing high intracellular uricase activities. Further analysis in simulated gastrointestinal conditions showed that Lactobacillus sp. OL-5, and Lactobacillus plantarum Dad-13 intracellular uricase, remained active after addition of gastric juice and duodenal juice. On the other hand, the intracellular of Lactobacillus plantarum Mut-7, and as well as both extracellular and membrane bond uricases of the three strains were not stable in gastric juice and duodenal juice. Thus based on uricase production and the stability in simulated gastrointestinal system, Lactobacillus plantarum Dad-13 is a potential strain for producing uricase. (C) All Rights Reserved

引用

页码：1661 / 1667

页数：7

共 32 条

[1]

Amirthanathan A., 2012, INT J MED BIOSCI, V1, P5

[2]

Azab E.A., 2005, Egypt. J. Biol, V7, P44

[3]

Bergmeyer H., 1974, Methods Enzymatic Anal, V3, P1205

[4] URIC-ACID DEGRADATION BY BACILLUS-FASTIDIOSUS STRAINS [J].

BONGAERTS, GPA ;

VOGELS, GD .

JOURNAL OF BACTERIOLOGY, 1976, 125 (02) :689-697

[5] Optimization of β-galactosidase production from lactic acid bacteria [J].

Carevic, Milica ;

Vukasinovic-Sekulic, Maja ;

Grbavcic, Sanja ;

Stojanovic, Marija ;

Mihailovic, Mladen ;

Dimitrijevic, Aleksandra ;

Bezbradica, Dejan .

HEMIJSKA INDUSTRIJA, 2015, 69 (03) :305-312

[6]

Cheng X, 2012, INTERNAL MED, V2, P2

[7] High pressure macromolecular crystallography:: The, 140-MPa crystal structure at 2.3 Å resolution of urate oxidase, a 135-kDa tetrameric assembly [J].

Colloc'h, N ;

Girard, E ;

Dhaussy, AC ;

Kahn, R ;

Ascone, I ;

Mezouar, M ;

Fourme, R .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2006, 1764 (03) :391-397

[8] Surviving the acid test: Responses of gram-positive bacteria to low pH [J].

Cotter, PD ;

Hill, C .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2003, 67 (03) :429-+

[9] Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes [J].

Dwivedi, Harinath ;

Agrawal, Kusum ;

Saraf, Shubhini A. .

E-JOURNAL OF CHEMISTRY, 2012, 9 (04) :2287-2296

[10] Improving the Stability and Activity of Oral Therapeutic Enzymes-Recent Advances and Perspectives [J].

Fuhrmann, Gregor ;

Leroux, Jean-Christophe .

PHARMACEUTICAL RESEARCH, 2014, 31 (05) :1099-1105

← 1 2 3 4 →