Phase-fluorimetry study on dielectric relaxation of human serum albumin

被引：13
作者：
Buzády, A
Erostyák, J
Somogyi, B
机构：
[1] Univ Pecs, Inst Phys, Dept Expt Phys, H-7624 Pecs, Hungary
[2] Univ Pecs, Dept Biophys, H-7625 Pecs, Hungary
[3] Univ Pecs, Jobin Yvon Mol Luminescence Reference Lab, H-7624 Pecs, Hungary
[4] Hungarian Acad Sci, Dept Biophys, Res Grp, H-7625 Pecs, Hungary
来源：
BIOPHYSICAL CHEMISTRY | 2000年 / 88卷 / 1-3期
关键词：
dielectric relaxation; human serum albumin; phase-fluorometry; time-emission matrix;
D O I：
10.1016/S0301-4622(00)00210-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The dielectric relaxation (DR) of human serum albumin (HSA) was studied by the method of phase-fluorometry, The protein environment of the single tryptophan in HSA shows a relatively low-speed DR of sub-ns characteristic time. This relaxation can be measured as a decaying red-shift of the time-resolved fluorescence emission spectra. The details of calculations of time-emission matrices (TEM) and comparison to the fluorescence data of the reference solution of N-acetyl-L-tryptophanamide (NATA) are also presented. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：153 / 163
页数：11
相关论文
共 35 条
1234