Single-Cell RNA Sequencing of Retina: New Looks for Gene Marker and Old Diseases

被引：8

作者：

Ying, Peixi ^{[1
]}

Huang, Chang ^{[2
,3
,4
,5
,6
]}

Wang, Yan ^{[7
]}

Guo, Xi ^{[8
]}

Cao, Yuchen ^{[1
]}

Zhang, Yuxi ^{[1
]}

Fu, Sheng ^{[9
]}

Chen, Lin ^{[10
]}

Yi, Guoguo ^{[11
]}

Fu, Min ^{[12
]}

机构：

[1] Southern Med Univ, Clin Sch 2, Guangzhou, Peoples R China

[2] Fudan Univ, Eye Inst, Eye & ENT Hosp, Shanghai, Peoples R China

[3] Fudan Univ, Dept Ophthalmol, Eye & ENT Hosp, Shanghai, Peoples R China

[4] Fudan Univ, NHC Key Lab Myopia, Shanghai, Peoples R China

[5] Chinese Acad Med Sci, Key Lab Myopia, Shanghai, Peoples R China

[6] Shanghai Key Lab Visual Impairment & Restorat, Shanghai, Peoples R China

[7] Shenzhen Univ, South China Hosp, Dept Ophthalmol, Hlth Sci Ctr, Shenzhen, Peoples R China

[8] Southern Med Univ, Med Coll Rehabil, Guangzhou, Peoples R China

[9] Univ South China, Hengyang, Peoples R China

[10] Southern Med Univ, Shenzhen Hosp, Dept Anesthesiol, Shenzhen, Peoples R China

[11] Sun Yat Sen Univ, Dept Ophthalmol, Affiliated Hosp 6, Guangzhou, Peoples R China

[12] Southern Med Univ, Zhujiang Hosp, Dept Ophthalmol, Guangzhou, Peoples R China

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2021年 / 8卷

关键词：

single-cell RNA sequencing; ScRNA-seq; retina; gene; retinal disease; HETEROGENEITY; SEQ; EXPRESSION; PATHWAYS; MODEL; IDENTIFICATION; PROGRESSION; ORGANOIDS; MICROGLIA; REVEALS;

D O I：

10.3389/fmolb.2021.699906

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The retina is composed of 11 types of cells, including neurons, glial cells and vascular bed cells. It contains five types of neurons, each with specific physiological, morphological, and molecular definitions. Currently, single-cell RNA sequencing (sRNA-seq) is emerging as one of the most powerful tools to reveal the complexity of the retina. The continuous discovery of retina-related gene targets plays an important role in helping us understand the nature of diseases. The revelation of new cell subpopulations can focus the occurrence and development of diseases on specific biological activities of specific cells. In addition sRNA-seq performs high-throughput sequencing analysis of epigenetics, transcriptome and genome at the single-cell level, with the advantages of high-throughput and highresolution. In this paper, we systematically review the development history of sRNA-seq technology, and summarize the new subtypes of retinal cells and some specific gene markers discovered by this technology. The progress in the diagnosis of retinal related diseases is also discussed.

引用

页数：9

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