Down-Regulation of FAD2-1 Gene Expression Alters Lysophospholipid Composition in the Endosperm of Rice Grain and Influences Starch Properties

被引:12
作者
Luo, Jixun [1 ,2 ]
Liu, Lei [3 ]
Konik-Rose, Christine [1 ]
Tian, Lijun [1 ,2 ]
Singh, Surinder [1 ]
Howitt, Crispin A. [1 ,2 ]
Li, Zhongyi [1 ]
Liu, Qing [1 ,2 ]
机构
[1] CSIRO, Agr & Food, Acton, ACT 2601, Australia
[2] CSIRO, Precis Hlth Future Sci Platform, Acton, ACT 2601, Australia
[3] Southern Cross Univ, Fac Sci & Engn, Southern Cross Plant Sci, Lismore, NSW 2480, Australia
关键词
high oleic rice; fatty acid; starch; amylose-lipid complex; lysophospolipid; phospholipid; AMYLOSE-INCLUSION COMPLEXES; PHYSICAL-PROPERTIES; FATTY-ACIDS; PHASE-TRANSITIONS; LIPID COMPLEXES; MAIZE; GRANULES; QUALITY;
D O I
10.3390/foods10061169
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Small quantities of lipids accumulate in the white rice grains. These are grouped into non-starch lipid and starch lipid fractions that affect starch properties through association with starch. Lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) are two major lipid classes in the two fractions. Using high-oleic rice grains, we investigated the fatty-acid composition in flour and starch by LC-MS and evaluated its impact on starch properties. In the wild-type grain, nearly 50% of fatty acids in LPC and LPE were palmitic acid (C16:0), over 20% linoleic acid (C18:2) and less than 10% oleic acid (C18:1). In the high-oleic rice grain, C18:1 increased at the expense of C18:2 and C16:0. The compositional changes in starch lipids suggest that LPC and LPE are transported to an amyloplast with an origin from endoplasmic reticulum-derived PC and PE during endosperm development. The high-dissociation temperature of the amylose-lipid complex (ALC) and restricted starch swelling power in the high-oleic rice starch indicates that the stability of the ALC involving C18:1 is higher than that of C18:2 and C16:0. This study provides insight into the lipid deposition and starch properties of rice grains with optimized fatty-acid composition.
引用
收藏
页数:10
相关论文
共 40 条
  • [1] Effects of lipids on enzymatic hydrolysis and physical properties of starch
    Ai, Yongfeng
    Hasjim, Jovin
    Jane, Jay-lin
    [J]. CARBOHYDRATE POLYMERS, 2013, 92 (01) : 120 - 127
  • [2] Understanding the control of acyl flux through the lipid metabolic network of plant oil biosynthesis
    Bates, Philip D.
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2016, 1861 (09): : 1214 - 1225
  • [3] A role for lipid trafficking in chloroplast biogenesis
    Benning, Christoph
    [J]. PROGRESS IN LIPID RESEARCH, 2008, 47 (05) : 381 - 389
  • [4] THERMAL CHARACTERIZATION OF RICE STARCHES - A POLYMERIC APPROACH TO PHASE-TRANSITIONS OF ANTIGRANULOCYTES STARCH
    BILIADERIS, CG
    PAGE, CM
    MAURICE, TJ
    JULIANO, BO
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1986, 34 (01) : 6 - 14
  • [5] Importance of phosphatidylcholine on the chloroplast surface
    Botella, Cesar
    Jouhet, Juliette
    Block, Maryse A.
    [J]. PROGRESS IN LIPID RESEARCH, 2017, 65 : 12 - 23
  • [6] Starch granules: structure and biosynthesis
    Buleon, A
    Colonna, P
    Planchot, V
    Ball, S
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1998, 23 (02) : 85 - 112
  • [7] Lipidomics reveals associations between rice quality traits
    Concepcion, Jeanaflor Crystal T.
    Calingacion, Mariafe
    Garson, Mary J.
    Fitzgerald, Melissa A.
    [J]. METABOLOMICS, 2020, 16 (05)
  • [8] PHYSICAL-PROPERTIES OF AMYLOSE MONOGLYCERIDE COMPLEXES
    ELIASSON, AC
    KROG, N
    [J]. JOURNAL OF CEREAL SCIENCE, 1985, 3 (03) : 239 - 248
  • [9] The Spatiotemporal Deposition of Lysophosphatidylcholine Within Starch Granules of Maize Endosperm and its Relationships to the Expression of Genes Involved in Endoplasmic Reticulum-Amyloplast Lipid Trafficking and Galactolipid Synthesis
    Gayral, Mathieu
    Fanuel, Mathieu
    Rogniaux, Helene
    Dalgalarrondo, Michele
    Elmorjani, Khalil
    Bakan, Benedicte
    Marion, Didier
    [J]. PLANT AND CELL PHYSIOLOGY, 2019, 60 (01) : 139 - 151
  • [10] Lipid Partitioning in Maize (Zea mays L.) Endosperm Highlights Relationships among Starch Lipids, Amylose, and Vitreousness
    Gayral, Mathieu
    Bakan, Benedicte
    Dalgalarrondo, Michele
    Elmorjani, Khalil
    Delluc, Caroline
    Brunet, Sylvie
    Linossier, Laurent
    Morel, Marie-Helene
    Marion, Dither
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2015, 63 (13) : 3551 - 3558