MOLECULAR IDENTIFICATION OF SOME SEEDLING OF DATE PALM (Phoenix dactylifera L.) MALES' TREES

被引:0
作者
El-Yazal, S. A. Seif [1 ]
Alharby, H. F. [4 ]
El-Yazal, M. A. Seif [2 ]
Hassan, G. M. [3 ]
Rady, M. M. [2 ]
机构
[1] Fayoum Univ, Fac Agr, Dept Hort, Al Fayyum 63514, Egypt
[2] Fayoum Univ, Fac Agr, Dept Bot, Al Fayyum 63514, Egypt
[3] Fayoum Univ, Fac Agr, Dept Genet, Al Fayyum 63514, Egypt
[4] King Abdulaziz Univ, Dept Biol Sci, Fac Sci, Jeddah 21589, Saudi Arabia
关键词
Date palm males; Genetic diversity; RAPD markers; GENETIC DIVERSITY; ISSR MARKERS; RAPD; GERMPLASM; CULTIVARS;
D O I
暂无
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The present investigation was conducted during the season of 2014 using five seedlings of date palm males selected from the evaluation of fifty male seedlings of date palm grown in Tamiya district, Fayoum Governorate, Egypt. To facilitate the selection and identification of good male pollinators for further using in breeding programs, the selection was carried out for promising males to be used to pollinate the females of date palm. The selected five male seedlings of date palm were analyzed using RAPD marker. Among a total of 77 bands obtained, 75 bands were polymorphic and the other 2 bands were monomorphic. Amplification size ranged between 280 and 2000 bp. UPGMA based cluster diagram showed that the all five different genotypes were grouped into two major clusters. Polymorphism percentage (96.4%) was indicated a high genetic diversity among the date palm studied.
引用
收藏
页码:1287 / 1294
页数:8
相关论文
共 31 条
  • [1] Abdulla M, 2010, AUST J CROP SCI, V4, P23
  • [2] Adawy S. S., 2004, ARAB J BIOTECH, V5, P225
  • [3] [Anonymous], 1996, Techniques in Plant Biology: The Practical Manual
  • [4] ARMSTRONG JS, 1994, RAPD DISTANCE PACKAG
  • [5] DAF optimization using Taguchi methods and the effect of thermal cycling parameters on DNA amplification
    Caetano-Anollés, G
    [J]. BIOTECHNIQUES, 1998, 25 (03) : 472 - +
  • [6] Croy E. J., 1993, PLANT NUCLEIC ACIDS
  • [7] Eissa1 E.A., 2009, Egypt. J. Genet. Cytol, V38, P269
  • [8] Hussein E. H. A, 2005, ARAB J BIOTECH, V8, P83
  • [9] Jaccard P., 1908, ETUDE COMP DISTRIBUT, V37, P547
  • [10] Jan HU, 2011, J MED PLANTS RES, V5, P823