Survey of Canadian human blood plasma for ochratoxin A

被引：91

作者：

Scott, PM ^{[1
]}

Kanhere, SR ^{[1
]}

Lau, BPY ^{[1
]}

Lewis, DA ^{[1
]}

Hayward, S ^{[1
]}

Ryan, JJ ^{[1
]}

Kuiper-Goodman, T ^{[1
]}

机构：

[1] Hlth Canada, Hlth Protect Branch, Food Directorate, Ottawa, ON K1A 0L2, Canada

来源：

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT | 1998年 / 15卷 / 05期

关键词：

ochratoxin A; mycotoxin; human exposure;

D O I：

10.1080/02652039809374681

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Blood plasma samples collected from 144 healthy volunteers in 16 locations across Canada in 1994 were analysed for ochratoxin A (OTA). The method of analysis included cleanup by C-18 solid phase extraction and immunoaffinity columns followed by liquid chromatography (LC) with fluorescence detection, which gave 86.5% (s.d. = 9.7)recovery (n = 31) of OTA (added at 2 ng/ml) with a detection limit of 0.15 ng/ml. The arithmetic mean concentration found in the plasma samples, corrected for volume of anticoagulant added was 0.88 ng/ml with a standard deviation of 0.35 ng/ml and a range of 0.29-2.37 ng/ml. Confirmation of identity of OTA was by methyl ester formation for 65 samples and by LC-tandem mass spectrometry for 17 samples (some of which were included in pooled samples). Statistical analysis, by ANOVA of the log OTA plasma concentrations, showed a highly significant effect due to location in Canada (p =<0.0001) but no effect due to age, sex or blood group of donors. The highest mean concentration was found in Winnipeg, significantly different (p = 0.05) by the Student-Newman-Keuls multiple range test from the lowest levels found in Toronto, Vancouver and Saint John.

引用

页码：555 / 562

页数：8

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