Sequence-independent and linear variation of oligonucleotide DNA binding stabilities

被引：27
作者：
Hoheisel, JD
机构：
[1] Molecular Genetic Genome Analysis, Deutsches Krebsforschungszentrum, D-69120 Heidelberg
来源：
NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 03期
关键词：
D O I：
10.1093/nar/24.3.430
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ability to equalize the DNA binding stability of comprehensive sets of oligonucleotides is imperative for the application of sequencing by hybridization technology. By substitution of ribonucleotides into an oligonucleotide composed of deoxyribonucleotides, and vice versa, the duplex stability of the oligonucleotide is changed linearly with the number of serial alternations of sugar configurations within the molecule. Since this effect occurs independently of the actual base sequence, any set of oligonucleotides could be adjusted to a defined level of binding stability.
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页码：430 / 432
页数：3
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