Immunofluorescence stimulated emission depletion microscopy

被引：107

作者：

Dyba, M ^{[1
]}

Jakobs, S ^{[1
]}

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37070 Gottingen, Germany

来源：

NATURE BIOTECHNOLOGY | 2003年 / 21卷 / 11期

关键词：

D O I：

10.1038/nbt897

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.

引用

页码：1303 / 1304

页数：2

共 5 条

[1] Focal spots of size λ/23 open up far-field florescence microscopy at 33 nm axial resolution -: art. no. 163901 [J].

Dyba, M ;

Hell, SW .

PHYSICAL REVIEW LETTERS, 2002, 88 (16) :4-163901

[2] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

[3]

Klar T. A., 2001, PHYS REV E, V64

[4] Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission [J].

Klar, TA ;

Jakobs, S ;

Dyba, M ;

Egner, A ;

Hell, SW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (15) :8206-8210

[5] CYTOPLASMIC MICROTUBULAR IMAGES IN GLUTARALDEHYDE-FIXED TISSUE-CULTURE CELLS BY ELECTRON-MICROSCOPY AND BY IMMUNOFLUORESCENCE MICROSCOPY [J].

WEBER, K ;

RATHKE, PC ;

OSBORN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (04) :1820-1824

← 1 →