Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

被引：25
作者：
Chou, Kai-Chieh [1 ]
Chen, Chun-Ting [2 ,3 ]
Cherng, Juin-Hong [1 ,4 ,5 ]
Li, Ming-Chia [6 ]
Wen, Chia-Cheng [7 ]
Hu, Sheng-, I [7 ]
Wang, Yi-Wen [1 ,5 ]
机构：
[1] Natl Def Med Ctr, Grad Inst Life Sci, Taipei 114, Taiwan
[2] Triserv Gen Hosp, Natl Def Med Ctr, Dept Internal Med, Div Gastroenterol & Hepatol,Penghu Branch, Taipei 114, Taiwan
[3] Triserv Gen Hosp, Natl Def Med Ctr, Dept Internal Med, Div Gastroenterol & Hepatol, Taipei 114, Taiwan
[4] Natl Def Med Ctr, Lab Adult Stem Cell & Tissue Regenerat, Taipei 114, Taiwan
[5] Natl Def Med Ctr, Dept & Grad Inst Biol & Anat, Taipei 114, Taiwan
[6] Natl Yang Ming Chiao Tung Univ, Ctr Intelligent Drug Syst & Smart Biodevices IDS2, Dept Biol Sci & Technol, Hsinchu 300, Taiwan
[7] Triserv Gen Hosp, Natl Def Med Ctr, Dept Surg, Div Colon & Rectal Surg, Taipei 114, Taiwan
来源：
POLYMERS | 2021年 / 13卷 / 20期
关键词：
burn injury; wound healing; beta-sheet silk fibroin; cell migration; integrin; extracellular matrix protein; Bombyx mori; EXTRACELLULAR-MATRIX; INTEGRIN BETA-1; BIOMATERIALS; FIBROBLASTS; COLLAGEN; METALLOPROTEINASES; SPECTROSCOPY; METASTASIS; EXPRESSION; SCAFFOLDS;
D O I：
10.3390/polym13203537
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Therapeutic dressings to enhance burn wound repair and regeneration are required. Silk fibroin (SF), a natural protein, induces cell migration and serves as a biomaterial in various dressings. SF dressings usually contain alpha-helices and beta-sheets. The former has been confirmed to improve cell proliferation and migration, but the wound healing effect and related mechanisms of beta-sheet SF remain unclear. We investigated the effects of beta-sheet SF in vivo and in vitro. Alcohol-treated alpha-helix SF transformed into the beta-sheet form, which promoted granulation formation and re-epithelialization when applied as lyophilized SF dressing (LSFD) in a rat burn model. Our in vitro results showed that beta-sheet SF increased human dermal fibroblast (HDF) migration and promoted the expression of extracellular matrix (ECM) proteins (fibronectin and type III collagen), matrix metalloproteinase-12, and the cell adhesion molecule, integrin beta 1, in rat granulation tissue and HDFs. This confirms the role of crosstalk between integrin beta 1 and ECM proteins in cell migration. In summary, we demonstrated that beta-sheet SF facilitates tissue regeneration by modulating cell adhesion molecules in dermal fibroblasts. LSFD could find clinical application for burn wound regeneration. Moreover, beta-sheet SF could be combined with anti-inflammatory materials, growth factors, or antibiotics to develop novel dressings.</p>
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页数：19
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