Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers

被引：152

作者：

Pang, TX ^{[1
]}

Su, XH ^{[1
]}

Wakabayashi, S ^{[1
]}

Shigekawa, R ^{[1
]}

机构：

[1] Natl Cardiovasc Ctr, Res Inst, Dept Mol Physiol, Suita, Osaka 5658565, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 20期

关键词：

D O I：

10.1074/jbc.M100296200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Na+/H+ exchangers (NHEs) comprise a family of transporters that catalyze cell functions such as regulation of the pH and volume of a cell and epithelial absorption of Na+ and bicarbonate. Ubiquitous calcineurin B homologous protein (CHP or p22) is co-localized and co-immunoprecipitated with expressed NHE1, NHE2, or NHE3 independently of its myristoylation and Ca2+ binding, and its binding site was identified as the jux-tamembrane region within the carboxyl-terminal cytoplasmic domain of exchangers. CHP binding-defective mutations of NHE1-3 or CHP depletion by injection of the competitive CHP-binding region of NHE1 into Xeno-pus oocytes resulted in a dramatic reduction (> 90%) in the Na+/H+ exchange activity. The data suggest that CHP serves as an essential cofactor, which supports the physiological activity of NHE family members.

引用

页码：17367 / 17372

页数：6

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