Production and characterization of agonistic monoclonal antibodies against chicken CD40

被引：6
作者：
Chen, Chang-Hsin [1 ]
Abi-Ghanem, Daad [1 ]
Njongmeta, Leo [3 ]
Bray, Jocelyn [3 ]
Mwangi, Waithaka [3 ]
Waghela, Suryakant D. [3 ]
McReynolds, Jackson L. [4 ]
Ing, Nancy H. [2 ]
Berghman, Luc R. [1 ]
机构：
[1] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA
[3] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX 77843 USA
[4] ARS, USDA, Agr Res Ctr, College Stn, TX 77845 USA
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2010年 / 34卷 / 11期
关键词：
Chicken; CD40; CD154; Monoclonal antibody; B-cells; Macrophages; HUMAN B-CELLS; NITRIC-OXIDE PRODUCTION; NECROSIS-FACTOR-ALPHA; IN-VIVO; IMMUNE-RESPONSES; BABESIA-BOVIS; LIGAND; ACTIVATION; MACROPHAGES; ANTIGEN;
D O I：
10.1016/j.dci.2010.06.014
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
CD40 is mainly expressed by professional antigen-presenting cells (APCs). Its ligand,CD40L, is transiently expressed on activated CD4(+) T-cells. CD40-CD40L interactions mediate T-cell help to APCs and provide crucial signals for affinity maturation and B-cell class switching. In mammals, agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming and expansion, increased antibody production and class switching. In this study, we describe agonistic anti-chicken CD40 mAb 2C5. This mAb detected CD40 on primary chicken B-cells and macrophages. DT40 B-cells, and HD11 macrophages, induced NO synthesis in HD11 macrophages, and stimulated DT40 B-cell proliferation. These observations demonstrated at least partial functional equivalence of 2C5 to chicken CD154. This mAb may therefore constitute a new tool to study the role of CD40 in the chicken immune system, and its agonistic effects suggest that it could also be used as an adjuvant. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1141 / +
页数：5
相关论文
共 41 条
[11]   IMMUNOCYTOCHEMISTRY AND IMMUNOBLOTTING OF AVIAN PROLACTINS USING POLYCLONAL AND MONOCLONAL-ANTIBODIES TOWARD A SYNTHETIC FRAGMENT OF CHICKEN PROLACTIN [J].
BERGHMAN, LR ;
GRAUWELS, L ;
VANHAMME, L ;
PROUDMAN, JA ;
FOIDART, A ;
BALTHAZART, J ;
VANDESANDE, F .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1992, 85 (03) :346-357
[12]   A SIMPLE METHOD FOR THE IMMUNOCYTOCHEMICAL PROCESSING OF LARGE NUMBERS OF FLOATING SECTIONS AND ITS APPLICATION IN SCREENING FOR MONOCLONAL-ANTIBODIES [J].
BERGHMAN, LR ;
GRAUWELS, L ;
VANHAMME, L ;
VANDESANDE, F .
JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 168 (02) :197-202
[13]   The role of CD40L in T cell-dependent nitric oxide production by murine macrophages [J].
Bingaman, AW ;
Pearson, TC ;
Larsen, CP .
TRANSPLANT IMMUNOLOGY, 2000, 8 (03) :195-202
[14]   ACTIVATION OF HUMAN B-CELLS MEDIATED THROUGH 2 DISTINCT CELL-SURFACE DIFFERENTIATION ANTIGENS, BP35 AND BP50 [J].
CLARK, EA ;
LEDBETTER, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4494-4498
[15]  
CLARK EA, 1989, J IMMUNOL, V143, P3873
[16]   Differential nitric oxide production by chicken immune cells [J].
Crippen, TL ;
Sheffield, CL ;
He, H ;
Lowry, VK ;
Kogut, MH .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2003, 27 (6-7) :603-610
[17]  
de Vinuesa CG, 1999, EUR J IMMUNOL, V29, P3216
[18]   CD40 activation in vivo overcomes peptide-induced peripheral cytotoxic T-lymphocyte tolerance and augments anti-tumor vaccine efficacy [J].
Diehl, L ;
den Boer, AT ;
Schoenberger, SP ;
van der Voort, EIH ;
Schumacher, TNM ;
Melief, CJM ;
Offringa, R ;
Toes, REM .
NATURE MEDICINE, 1999, 5 (07) :774-779
[19]   Enhancement of T cell-independent immune responses in vivo by CD40 antibodies [J].
Dullforce, P ;
Sutton, DC ;
Heath, AW .
NATURE MEDICINE, 1998, 4 (01) :88-91
[20]   CD40 antibody evokes a cytotoxic T-cell response that eradicates lymphoma and bypasses T-cell help [J].
French, RR ;
Chan, HTC ;
Tutt, AL ;
Glennie, MJ .
NATURE MEDICINE, 1999, 5 (05) :548-553
12345